Biophysical techniques /
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| Author / Creator: | Campbell, Iain D., author. |
|---|---|
| Imprint: | Oxford : Oxford University Press, [2012] ©2012 |
| Description: | 1 online resource (ix, 353 pages) : illustrations (some color) |
| Language: | English |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11303544 |
Table of Contents:
- Cover; Contents; Some frequently used abbreviations; 1 Introduction; What are "biophysical techniques"?; What questions can biophysical techniques answer?; Which technique to use?; Organization of this book; 2 Molecular principles; 2.1 Molecules and interactions; Introduction; Box 2.1 Atoms and elements; Box 2.2 Kinetic model of gases; Non-covalent interactions; Box 2.3 Electrostatics, dielectrics, and polarity; Box 2.4 Molecular biology tools and base pairing; Binding; 3 Transport and heat; 3.1 Diffusion, osmosis, viscosity, and friction; Introduction; Diffusion; Osmosis.
- Application of a force to a molecule in solutionViscosity; Box 3.1 The frictional coeffi cient, f, depends on molecular shape; 3.2 Analytical centrifugation; Introduction; Some basic principles of sedimentation; Sedimentation velocity; Sedimentation equilibrium; Density gradient sedimentation; 3.3 Chromatography; Introduction; Theory; Chromatography techniques; Quantitative chromatography; 3.4 Electrophoresis; Introduction; Theory; Experimental; Some electrophoresis systems; 3.5 Mass spectrometry; Introduction; Ionization; Ion sorting/analysis; Applications of mass spectrometry.
- 3.6 ElectrophysiologyIntroduction; Membrane potential; Action potentials; Experimental; Propagation of an action potential in a neuron; 3.7 Calorimetry; Introduction; Isothermal titration calorimetry; Differential scanning calorimetry; Box 3.2 Heat capacity; 4 Scattering, refraction, and diffraction; 4.1 Scattering of radiation; Introduction; Scattering theory; Box 4.1 Weight average molecular weight; Turbidity; Solution scattering and molecular shape; Box 4.2 Radii of gyration, R[sub(G)], and hydration, R[sub(H)]; Dynamic light scattering; Box 4.3 Correlation functions.
- 4.2 Refraction, evanescent waves, and plasmonsIntroduction; Box 4.4 Classical optics; Evanescent waves; Surface plasmon resonance; Box 4.5 The streptavidin/biotin complex; 4.3 Diffraction; Introduction; Principles of diff raction; Box 4.6 Single particle imaging with X-ray laser; Diffraction experiments; Interpretation of diffraction data; Other crystallography techniques; Achievements of crystallography; 5 Electronic and vibrational spectroscopy; 5.1 Introduction to absorption and emission spectra; Introduction; Energy states; Absorption.
- Box 5.1 Transition dipole moments and transition probabilityEmission; Box 5.2 The laser; 5.2 Infrared and Raman spectroscopy; Introduction; IR spectra and applications; Raman scattering; Applications of Raman spectroscopy; 5.3 Ultraviolet/visible spectroscopy; Introduction; Measurement of electronic spectra; Electronic energy levels and transitions; Absorption properties of some key chromophores; Applications of UV/visible spectra; Box 5.3 Isosbestic points; Properties associated with the direction of the transition dipole moment; Monitoring rapid reactions; 5.4 Optical activity; Introduction.