Microbial steroids : methods and protocols /

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Bibliographic Details
Imprint:	New York : Humana Press : Springer, ©2017.
Description:	1 online resource (xi, 373 pages) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology, 1940-6029 ; 1645 Methods in molecular biology (Clifton, N.J.) ; v. 1645.
Subject:	Steroids. Biotransformation (Metabolism) Steroids Biotransformation Microbiological Techniques Biotransformation (Metabolism) Steroids Laboratory Manual Laboratory manuals Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11322993

Hidden Bibliographic Details
Other authors / contributors:	Barredo, José-Luis, editor. Herraiz, Ignacio, editor.
ISBN:	9781493971831 1493971832 9781493971824 1493971824
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. Online resource; title from PDF title page (SpringerProtocol, viewed July 17, 2017).
Summary:	This volume provides practical experimental laboratory protocols for a wide range of steroid bioconversions. The chapters in this book cover topics such as bioconversions and chemical synthesis pathways; strain characterization; bioconversion from sterols to androstenedione and androstadienedione; steroid hydroxylations; biocatalysis; and downstream processes to purify steroid intermediates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Microbial Steroids: Methods and Protocols is a valuable resource for laboratory and industrial professionals. It is also useful for graduate students studying biotechnology, microbiology, genetics, and molecular biology.
Other form:	Print version: Microbial steroids. New York : Humana, 2017 9781493971824
Standard no.:	10.1007/978-1-4939-7183-1

Table of Contents:

Steroid Bioconversions
Chemical Pathways of Corticosteroids, Industrial Synthesis from Sapogenins
Genome Sequencing of Steroid Producing Bacteria with Illumina Technology
Genome Sequencing of Steroid Producing Bacteria using Ion Torrent Technology and a Reference Genome
RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics
Intra- and Extra-Cellular Proteome Analyses of Steroid-Producer Mycobacteria
Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacteriumneoaurum NRRL B-3805
Identification and Characterization of the genes and Enzymes Belonging to the Bile Acid Catabolic Pathway in Pseudomonas
Selection of Biodegrading Phytosterol Strains
Stigmasterol Removal by an Aerobic Treatment System
Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3, 17-Dione
?-Sitosterol Bioconversion to Androstenedione in Microtiter Plates
Bioconversion of Phytosterols into Androstenedione by Mycobacterium
Scale-Up of Phytosterols Bioconversion into Androstenedione
Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331
Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1, 4-Diene-3, 17-Dione (ADD)
Laboratory-Scale Hydroxylation of Steroids by P450BM3 Variants
Obtaining of 11?-Hydroxy and Rost-4-ene-3, 17-Dione from Natural Sterols
Steroid 11-Alfa-Hydroxylation by the Fungi Aspergillus nidulans and Aspergillus ochraceus
Biotransformation of DHEA into 7?, 15?-diOH-DHEA
Stabilization of Enzymes by Using Thermophiles
Biocatalysis of Steroids with Mycobacterium Sp. in Aqueous and Organic Media
Downstream Process Synthesis for Microbial Steroids
Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin Layer Chromatography (TLC)
A Ligand-Binding Assay to Measure the Affinity and Specificity of Sterol-Binding Proteins In Vitro.

Microbial steroids : methods and protocols /

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