Skeletal muscle development /

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Bibliographic Details
Imprint:	New York, NY : Humana Press, 2017.
Description:	1 online resource (xii, 228 pages) : illustrations (some color).
Language:	English
Series:	Methods in molecular biology, 1940-6029 ; volume 1668 Methods in molecular biology (Clifton, N.J.) ; v. 1668,
Subject:	Myogenesis -- Laboratory manuals. Skeleton -- Muscles -- Growth -- Laboratory manuals. Myogenesis. Muscle, Skeletal -- growth & development. Muscle Development -- genetics. Muscle Fibers, Skeletal -- cytology. Myoblasts, Skeletal -- cytology. Sequence Analysis. Laboratory manuals. Laboratory Manual.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/11350104

Hidden Bibliographic Details
Other authors / contributors:	Ryall, James G. editor.
ISBN:	9781493972838 1493972839 9781493972821 1493972820 9871493972838 (eBook)
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. Includes bibliographical references and index. Online resource; title from PDF title page (SpringerLink, viewed August 31, 2017).
Summary:	This detailed volume presents a wide range of techniques that go beyond the standard assays typically used to assess Myogenesis. The content included addresses assays to analyze skeletal muscle gene expression, proliferating muscle cells, the process of myoblast fusion, muscle development (in vivo), as well as muscle repair. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Skeletal Muscle Development serves as an ideal guide to the study of this highly complex yet carefully regulated process.>.
Other form:	Original 1493972820 9781493972821
Standard no.:	10.1007/978-1-4939-7283-8

Table of Contents:

Bisulfite Sequencing for DNA Methylation Analysis of Primary Muscle Stem Cells
Whole Genome Chromatin IP-Sequencing (ChIP-Seq) in Skeletal Muscle Cells
Analysis of RNA Expression in Adult Zebrafish Skeletal Muscle
Chick Somite Electroporation for Gene Function Studies
Targeted Lipidomic Analysis of Myoblasts by GC-MS and LC-MS/MS
Measuring Mitochondrial Substrate Utilization in Skeletal Muscle Stem Cells
Microcontact-Printed Hydrogel Microwell Arrays for Clonal Muscle Stem Cell Cultures
Isolation, Culture, and Differentiation of Fibro/Adipogenic Progenitors (FAPs) from Skeletal Muscle
Human Satellite Cell Isolation and Xenotransplantation
Application of Split-GFP Reassembly Assay to Study Myogenesis and Myofusion In Vitro
Myogenic Maturation by Optical-Training in Cultured Skeletal Muscle Cells
Fabrication of Micromolded Gelatin Hydrogels for Long-Term Culture of Aligned Skeletal Myotubes
Quantification of Embryonic Myofiber Development by Immunofluorescence
How to Wire the Diaphragm: Wholemount Staining Methods to Analyze Mammalian Respiratory Innervation
Membrane Repair Assay for Human Skeletal Muscle Cells
Cryoinjury Model for Tissue Injury and Repair in Bioengineered Human Striated Muscle.

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