Plant germline development : methods and protocols /
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| Imprint: | New York, NY : Humana Press : Springer, ©2017. |
|---|---|
| Description: | 1 online resource (xiii, 422 pages) : illustrations (some color) |
| Language: | English |
| Series: | Methods in molecular biology ; v. 1669 Methods in molecular biology (Clifton, N.J.) ; v. 1669. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/11361599 |
Table of Contents:
- Introduction to male germline development / Hannes Vogler, Ueli Grossniklaus, and Afif Hedhly
- Apomixis : engineering the ability to harness hybrid vigor in crop plants / Joann A. Conner and Peggy Ozias-Akins
- 3D imaging of whole-mount ovules at cellular resolution to study female germline development in rice / Ethel Mendocilla-Sato, Wenjing She, and Celia Baroux
- Live-cell imaging of F-actin dynamics during fertilization in Arabidopsis thaliana / Daichi Susaki, Daisuke Maruyama, Ramesh Yelagandula, Frederic Berger, and Tomokazu Kawashima
- Development and observation of mature megagametophyte cell-specific fluorescent markers / Mark A. Chamberlin and Shai J. Lawit
- Analysis of fluorescent reporter activity in the male germline during pollen development by confocal microscopy / David Twell and Lynette Brownfield
- In vivo ploidy determination of Arabidopsis thaliana male and female gametophytes / Burcu Nur Keceli, Nico De Storme, and Danny Geelen
- Staining and clearing of arabidopsis reproductive tissue for imaging of fluorescent proteins / Daniel Slane, Patrick Burgel, and Martin Bayer
- Live-cell imaging of auxin and cytokinin signaling in maize female gametophytes / Antony M. Chettoor and Matthew M.S. Evans
- Imaging Ca2+ dynamics in wild-type and NADPH oxidase-deficient mutant pollen tubes with yellow cameleon and confocal laser scanning microscopy / Christina Maria Franck, Jens Westermann, and Aurelien Boisson-Dernier
- Immunolocalization of AGPs and pectins in Quercus suber gametophytic structures / Mario Luıs da Costa, Ana Lucia Lopes, Maria Isabel Amorim, and Sılvia Coimbra
- Optimization of cell spreading and image quality for the study of chromosomes in plant tissues / Laksana Kantama, Erik Wijnker, and Hans de Jong
- Whole mount RNA-FISH on ovules and developing seeds / Andrea Bleckmann and Thomas Dresselhaus
- Analysis of peroxisome biogenesis in pollen by confocal microscopy and transmission electron microscopy / Peng-Fei Jia, Hong-Ju Li, and Wei-Cai Yang
- Transmission electron microscopy (TEM) to study histology of pollen and pollen tubes / Peng-Fei Jia, Hong-Ju Li, and Wei-Cai Yang
- Isolation of Arabidopsis pollen, sperm cells, and vegetative nuclei by fluorescence-activated cell sorting (FACS) / Mario R. Santos, Claudia Bispo, and Jorg D. Becker
- Isolation of rice sperm cells for transcriptional profiling / Scott D. Russell, Daniel S. Jones, Sarah Anderson, Xinkun Wang, Venkatesan Sundaresan, and Xiaoping Gou
- Manual isolation of living cells from the Arabidopsis thaliana female gametophyte by micromanipulation / Maria Englhart, Lucija Soljic, and Stefanie Sprunck
- Isolation and detection of small RNAs from pollen / German Martinez
- Analysis of proteins enriched in rice gamete / Takashi Okamoto
- Phosphoprotein enrichment from tobacco mature pollen crude protein extract / Jan Fıla and David Honys-- Identification of cis-regulatory modules that function in the male germline of flowering plants / Benjamin Peters, Jack Aidley, Murray Cadzow, David Twell, and Lynette Brownfield
- RNA-seq data analysis : from raw data quality control to differential expression analysis / Weihong Qi, Ralph Schlapbach, and Hubert Rehrauer
- RNA-seq data analysis protocol : combining in-house and publicly available data / Marc W. Schmid
- Impedance flow cytometry as a tool to analyze microspore and pollen quality / Iris Heidmann and Marco Di Berardino
- Agrobacterium-mediated Sorghum transformation / Emily Wu and Zuo-Yu Zhao
- Use of the Cas9 orthologs from Streptococcus thermophilus and Staphylococcus aureus for non-homologous end-joining mediated site-specific mutagenesis in Arabidopsis thaliana / Jeannette Steinert, Carla Schmidt, and Holger Puchta
- Transgenic reproductive cell ablation / Shai J. Lawit and Mark A. Chamberlin
- In vivo reporters for protein half-life / Pavel Reichman and Nico Dissmeyer
- Conditional modulation of biological processes by low-temperature degrons / Nico Dissmeyer.