A clinician's guide to sperm DNA and chromatin damage /

A clinician's guide to sperm DNA and chromatin damage /

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Bibliographic Details
Imprint:Cham : Springer, 2018.
Description:1 online resource
Language:English
Subject:
DNA damage.
Chromatin.
DNA Damage.
Chromatin.
DNA damage.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11543857
Hidden Bibliographic Details
Other authors / contributors:Zini, Armand, editor.
Agarwal, Ashok (Physician), editor.
ISBN:9783319718156
3319718150
9783319718149
3319718142
Digital file characteristics:text file
PDF
Notes:Online resource; title from PDF title page (EBSCO, viewed March 13, 2018).
Summary:This comprehensive, up-to-date text, which brings together the key practical elements of the rapidly evolving field of sperm DNA and chromatin abnormalities, is divided thematically into five main sections. Part I discusses human sperm chromatin structure and nuclear architecture, while part II presents laboratory evaluation of sperm DNA damage, including SCSA, SCD, TUNEL and Comet assays, and cytochemical tests. Biological and clinical factors in the etiology of sperm DNA damage are discussed in part III, including oxidative stress, abortive apoptosis, cancer, and environmental and lifestyle factors. Part IV presents clinical studies on the utility of sperm DNA damage tests, both with natural and ART-assisted pregnancies, and debates the clinical utility of such tests. Finally, part V discusses current treatment options, such as antioxidant therapy, varicocelectomy, advanced sperm processing techniques and the use of testicular sperm. We are now beginning to better understand the unique organization of the sperm chromatin, as well as the nature and etiology of sperm DNA damage. Written and edited by worldwide experts in andrology, A Clinician's Guide to Sperm DNA and Chromatin Damage is an excellent resource for reproductive medicine and REI specialists, urologists, reproductive biologists and any professional working with the infertile male.
Other form:Print version: Clinician's guide to sperm DNA and chromatin damage. Cham : Springer, 2018 3319718142 9783319718149
Standard no.:10.1007/978-3-319-71815-6
Table of Contents:
  • Intro; Foreword; Preface; Contents; Editors; Contributors; Part I: Human Sperm Chromatin Structure; Chapter 1: Sperm Chromatin: An Overview; 1.1 Sperm Chromatin: The First 50 Years; 1.2 Spermatogenesis: Terminal Differentiation and Reprogramming of the Testicular Cell Genome; 1.3 Chromatin Reorganization in Maturing Spermatids; 1.4 Protamines P1 and P2; 1.5 Protamine-DNA Interactions and Structure of the Complex; 1.6 Higher-Ordered Organization of Chromatin in Mature Sperm; 1.7 Preservation of DNA Domains and Nuclear Matrix Associations
  • 1.8 Reactivation of Paternal Chromatin Following Sperm-­Oocyte Fusion1.9 Consequences of Disrupting Sperm Chromatin Remodeling; References; Chapter 2: Sperm Nucleoproteins (Histones and Protamines); 2.1 Introduction; 2.2 Nucleoprotamine Complex in Sperm; 2.2.1 Protamine Post-translational Modifications; 2.2.2 Protamine Alterations in Infertile Patients; 2.3 Nucleohistone Complex in Sperm; 2.3.1 Histone Variants; 2.3.2 Histone Post-translational Modifications; 2.3.3 Histone-Bound Sperm Chromatin; 2.3.4 Histone Alterations and Male Infertility; 2.4 Concluding Remarks; References
  • Chapter 3: Sperm Nuclear Architecture3.1 Introduction; 3.2 A Model; 3.3 Sperm Nuclear Matrix; 3.4 Sperm Chromosomes; 3.5 Conclusions; References; Part II: Laboratory Evaluation of Sperm Chromatin and DNA Damage; Chapter 4: Sperm Chromatin Structure Assay (SCSA®): Evolution from Origin to Clinical Utility; 4.1 Origin, Standardization, and Verification of the SCSA Test as Marker of Male Sub-/Infertility; 4.1.1 Origin of the SCSA Test; 4.1.2 Standardization of the SCSA Test: Changes to the Finalized and Federal Registered Protocol; 4.1.2.1 Problems with the Heated Sperm Nuclei Protocol
  • 4.1.2.2 Low pH to Denature DNA at DNA Break Sites4.1.3 Biochemistry of Acridine Orange (AO) and Sperm DNA Interactions of the SCSA Test; 4.1.4 Does the SCSA Test Measure Potential or Existing Sperm DNA Strand Breaks?; 4.1.5 Change in SCSA Terminology; 4.1.6 Clinical Report; 4.1.7 Relationship Between Sperm DNA Fragmentation Data and Classical Semen Parameters; 4.2 Biochemical Characteristics of SCSA-Defined Sperm Populations; 4.2.1 FCM Sorted SCSA Populations to Analyze Sperm Nuclear Morphology; 4.2.2 Characteristics of HDS Population: New Emphasis for the ART Lab
  • 4.3 Validation of SCSA Clinical Thresholds4.3.1 Humans; 4.3.2 Animals; 4.3.2.1 Bulls; 4.3.2.2 Boars; 4.4 Validation of the SCSA Test for Precision and Accuracy; 4.4.1 Invaluable Use of Flow Cytometry; 4.4.2 Repeatability of SCSA Data; 4.4.2.1 Within Human Donors Over Time; 4.4.2.2 SCSA Data Using Different Flow Cytometers Internationally on Sperm from Eight Different Mammalian Species; 4.4.2.3 Comparisons Between Measurements of Aliquots of Human Patient Semen Samples on Three Continents; 4.5 SCSA Data as Related to Male Age, a Very Important Infertility Issue

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