T-cell motility : methods and protocols /

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Bibliographic Details
Imprint:New York : Humana Press : Springer, [2019]
Description:1 online resource (x, 158 pages) : illustrations (chiefly color)
Language:English
Series:Methods in molecular biology, 1940-6029 ; volume 1930
Methods in molecular biology (Clifton, N.J.) ; v. 1930.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11780923
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Other authors / contributors:Verma, Navin Kumar, editor.
ISBN:9781493990368
1493990365
9781493990351
1493990357
Notes:Includes bibliographical references and index.
Online resource; title from PDF title page (SpringerLink, viewed January 7, 2019).
Other form:Print version: T-cell motility. New York : Springer, ©2019 9781493990351
Table of Contents:
  • Introduction to LFA-1/ICAM-1 interactions in T-cell motility / Navin Kumar Verma and Dermot Kelleher
  • Isolation of human peripheral blood T-lymphocytes / Atish Kizhakeyil, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, and Navin Kumar Verma
  • Laboratory model to study T-cell motility / Navin Kumar Verma, Shyam Mohan Palapetta, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, Atish Kizhakeyil, and Dermot Kelleher
  • Quantification of T-cell migratory phenotypes using high-content analysis / Aik Seng Ng, Seow Theng Ong, Dermot Kelleher, and Navin Kumar Verma
  • Live cell imaging and analysis to capture T-cell motility in real-time / Seow Theng Ong and Navin Kumar Verma
  • Three-dimensional structured illumination microscopy (3D-SIM) to dissect signaling cross-talks in motile T-cells / Seow Theng Ong, Graham D. Wright, and Navin Kumar Verma
  • Real-time impedance-based detection of LFA-1-stimulated T-cell transwell chemotaxis / Praseetha Prasannan and Navin Kumar Verma
  • Quantitative real-time PCR for Evaluating Transcriptional Changes in T-Lymphocytes / Atish Kizhakeyil and Navin Kumar Verma
  • GapmeR-mediated gene silencing in motile T-cells / Mobashar Hussain Urf Turabe Fazil, Seow Theng Ong, Madhavi Latha Somaraju Chalasani, Atish Kizhakeyil, and Navin Kumar Verma
  • Live imaging of resident T-cell migration in human lymphoid tissue slices using confocal microscopy / Emmanuel Donnadieu, Yvonne Michel, and Martin-Leo Hansmann
  • Phosphoprotein enrichment for protein analysis in motile T-lymphocytes / Praseetha Prasannan, Rathi Saravanan, and Navin Kumar Verma
  • Immunometabolomic phenotyping of motile T-cells / Madhavi Latha Somaraju Chalasani, Gaelin Looi Wen Dong, and Navin Kumar Verma
  • Profiling activity of cellular kinases in migrating T-cells / Chandra Sekhar Chirumamilla, Mobashar Hussain Urf Turabe Fazil, Claudina Perez-Novo, Savithri Rangarajan, Rik de Wijn, Padma Ramireddy, Navin Kumar Verma, and WimVanden Berghe
  • Biotinylation assay to determine LFA-1 recycling in motile T-lymphocytes / Malin Samuelsson and Lena M. Svensson
  • Protocol to study T-cell signaling in an immune synapse by microscopy / Mobashar Hussain Urf Turabe Fazil, Pankaj Kumar, and Navin Kumar Verma
  • Enzyme-linked immunosorbent assay for T-cell dependent immunogenicity assessment of therapeutic peptides / Madhavi Latha Somaraju Chalasani, Rajamani Lakshminarayanan, and Navin Kumar Verma
  • Modified intravital microscopy to assess vascular health and T-cell motility / Geoffrey W. Payne, Kevin Mitchell, and Stephanie L. Sellers
  • Computational analysis of protein-protein interactions in motile T-cells / Sunil Kumar, Mobashar Hussain Urf Turabe Fazil, Khurshid Ahmad, Minaketan Tripathy, Jagath Chandana Rajapakse, and Navin Kumar Verma.