Mycotoxin protocols /

Saved in:
Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2001.
Description:1 online resource (xii, 244 pages) : illustrations
Language:English
Series:Methods in molecular biology ; 157
Methods in molecular biology (Clifton, N.J.) ; v. 157.
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11929867
Hidden Bibliographic Details
Other authors / contributors:Trucksess, Mary W.
Pohland, Albert E.
ISBN:9781592590643
1592590640
0896036235
9780896036239
1280820551
9781280820557
9786610820559
6610820554
9781489938725
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:Mycotoxins, common food contaminants produced by molds, are associated with a broad range of serious toxic effects, including carcinogenicity, neurotoxicity, and reproductive and developmental toxicity, are subject to regulatory restrictions in more than 80 countries. In Mycotoxin Protocols, a panel of accomplished scientists describe their innovative, cutting-edge methods for determining the levels of various mycotoxins in foods and feeds. Almost half of the methods presented involve molecular-based immunochemical or immunochemical/chromatographic techniques. The necessary equipment, reagents, and procedures are given in great detail for the analysis of a wide variety of significant mycotoxins, including aflatoxins, aflatoxin M1, cyclopiazonic acid, ochratoxin A, trichothcenes, moniliformin, fumonisins, zearalenone, Stachybotrys toxins, citrinin, patulin, ergot alkaloids, and Alternaria toxins. General techniques for mycotoxin analyses, sampling procedures for collecting representative test samples, isolation techniques, and techniques for the detection and identification of toxins and impurities are also included. Up-to-date and highly practical, Mycotoxin Protocols provides a comprehensive collection of the latest bioanalytical techniques for determining mycotoxins in foods and feeds.
Other form:Print version: Mycotoxin protocols. Totowa, N.J. : Humana Press, ©2001 0896036235 9780896036239
Standard no.:10.1385/1592590640.
Table of Contents:
  • pt. 1. General techniques
  • Mycotoxin method evaluation: an introduction
  • Sampling techniques
  • Preparatory isolation of mycotoxins from solid phase fungal cultures
  • Preparation of mycotoxin standards
  • Electrospray mass spectrometry for mycotoxin detection and purity analysis
  • pt. 2. Methods for aspergillus toxins
  • Measurement of aflatoxins using capillary electrophoresis
  • Immunochemical method for cyclopiazonic acid
  • Immunochemical method for ochratoxin A
  • pt. 3. Methods for fusarium toxins
  • Solution fluorometric method for deoxynivalenol in grains
  • Chromatographic method for trichothecenes
  • Chromatographic method for the determination of the mycotoxin moniliformin in corn
  • Liquid chromatographic method for fumonsins in corn
  • Enzyme-linked immunosorbent assays of zearalenone using polyclonal, monoclonal, and recombinant antibiodies
  • pt. 4. Methods for other fungal toxins
  • Chromatographic method for stachybotrys toxins
  • Immunochemical method for citrinin
  • Solid phase extraction method for patulin in apple juide and unfiltered apple juice
  • Liquid chromatographic method for the determination of ergot alkaloids in cereal grains
  • Chromatographic method for alternaria toxins in apple juice
  • Index.