Capillary electrophoresis of carbohydrates /

Capillary electrophoresis of carbohydrates /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, ©2003.
Description:1 online resource (xiv, 318 pages) : illustrations
Language:English
Series:Methods in molecular biology ; v. 213
Methods in molecular biology (Clifton, N.J.) ; v. 213.
Subject:
Carbohydrates -- Separation.
Capillary electrophoresis.
Carbohydrates -- analysis.
Electrophoresis, Capillary -- methods.
Capillary electrophoresis.
Laboratory Manual.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/11929868
Hidden Bibliographic Details
Other authors / contributors:Thibault, Pierre.
Honda, Susumu.
ISBN:9781592592944
1592592945
0896038262
9780896038264
1280830778
9781280830778
9786610830770
6610830770
9781617372063
Notes:Includes bibliographical references and index.
English.
Print version record.
Summary:Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated mono- and oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. Written in step-by-step detail to ensure successful experimental results, these readily reproducible protocols provide methods for sample preparation and analysis of mono- and oligosaccharides, glycoproteins, and glycoconjugates. Different CE separation formats, including capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), capillary gel electrophoresis (CGE), and micellar electrokinetic chromatography (MEKC), are presented for different types of applications, such as the analysis of glycoforms, the separation of enantiomers, and the resolution of oligosaccharides. The separation of native derivatized carbohydrates is presented using UV, fluorescence, and mass spectrometric detection. Two other applications demonstrate the use of CE as an assay method for monitoring glycosyltransferase activity and for determining the association constant of carbohydrate-protein interaction. A useful appendix describes the structures of the most commonly encountered carbohydrate residues and oligosaccharides from mammalian and bacterial origins. Each protocol contains detailed information on reagents, apparatus, and valuable comments and notes on methods. Timely and highly practical, Capillary Electrophoresis of Carbohydrates provides both novice and experienced CE analysts with powerful tools for the successful separation of complex carbohydrates by capillary electrophoresis.
Other form:Print version: Capillary electrophoresis of carbohydrates. Totowa, N.J. : Humana Press, ©2003 0896038262 9780896038264
Standard no.:10.1385/1592592945.
Table of Contents:
  • Dedication
  • Preface
  • Contributors
  • Part I.. Introduction
  • 1. Structural and Functional Diversity of Glycoconjugates: A Formidable Challenge to the Glycoanalyst
  • Part II.. Sample Preparation Methods
  • 2. Chemical and Enzymatic Release of Glycans from Glycoproteins
  • 3. Derivatization of Carbohydrates
  • 4. Preparation of Oligosaccharides from Sulfated Glycosaminoglycans Using Bacterial Enzymes
  • Part III.. Analysis of Mono- and Oligosaccharides
  • 5. Monosaccharide Analysis by Capillary Electrophoresis
  • 6. Separation of Oligosaccharides by Capillary Electrophoresis Using Buffer Modifiers
  • 7. Characterization of Oligosaccharides from Starch, Dextran, Cellulose, and Glycoproteins by Capillary Electrophoresis
  • 8. Affinity Capillary Electrophoresis of Oligosaccharides
  • 9. Characterization of Glycosaminoglycans by Capillary Electrophoresis
  • Part IV.. Analysis of Glycoconjugates and the Component Monosaccharides
  • 10. Analysis of 2-Aminoacridone-Derivatized Complex Oligosaccharides Using Micellar Electrokinetic Capillary Chromatography and Laser-Induced Fluorescence Detection
  • 11. Separation of Protein Glycoforms by Capillary Electrophoresis
  • 12. Separation of Glycoproteins by Capillary Isoelectric Focusing
  • 13. Glycoprotein Analysis by Capillary Electrophoresis-Electrospray Mass Spectrometry
  • 14. Analysis of Bacterial Glycolipids by Capillary Electrophoresis-Electrospray Mass Spectrometry: Haemophilus influenzae and Neisseria meningitidis Lipopolysaccharides
  • Part V.. Specific Applications
  • 15. Capillary Electrophoresis as an Assay Method for Monitoring Glycosyltransferase Activity
  • 16. Determination of Association Constant of Carbohydrate-Protein Interaction
  • Appendix
  • Structures of Carbohydrates Found in Animals and Bacteria
  • Index

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