Molecular biomethods handbook /
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| Edition: | 2nd ed. |
|---|---|
| Imprint: | Totowa, NJ : Humana Press, ©2008. |
| Description: | 1 online resource (xx, 1124 pages) : illustrations |
| Language: | English |
| Series: | Methods in Molecular Biology, v. 452 ; v. v. 452 Methods in molecular biology (Clifton, N.J.) ; v. 452. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/12039465 |
Table of Contents:
- Nucleic acid methods: The manipulation of nucleic acids : basic tools and techniques / Gayle Corkill and Ralph Rapley. Restriction enzymes : tools in clinical research / Gareth J.S. Jenkins. Principles and medical applications of the polymerase chain reaction / Bimal D.M. Theophilus. Probe design, production, and applications / Marilena Aquino de Muro. Southern blotting as a diagnostic method / Bronwen M. Harvey and Pirkko Soundy. Capillary electrophoresis of DNA : biomedical applications / Beatriz Sanchez-Vega. Denaturing high-performance liquid chromatography (DHPLC) for nucleic acid analysis / Kim Hung Leung and Shea Ping Yip. Denaturing gradient gel electrophoresis (DGGE) / Jeroen H. Roelfsema and Dorien J.M. Peters. Single strand conformation polymorphism (SSCP) analysis / Kim Hung Leung and Shea Ping Yip. Randomly amplified polymorphic DNA (RAPD) : a useful tool for genomic characterization of different organisms / Lúcia Maria da Cunha Galvão and Eliane Lages-Silva. Quantification of mRNA using real time RT-PCR : the SYBR solution / David Sugden and Patricia de Winter. Quantitative analysis of DNA sequences by PCR and solid-phase minisequencing / Anu Suomalainen and Ann-Christine Syvänen. Multiplex amplifiable probe hybridization (MAPH) / Carolina Sismani, Ludmila Kousoulidou, and Philippos C. Patsalis. Gene expression profiling / Arnis Druka, Robbie Waugh, and Pete Hedley. Comparative genomic hybridization in clinical and medical research / Peng-Hui Wang, Yann-Jang Chen, and Chi-Hung Lin. Subtractive hybridization / Craig Winstanley. Fluorescence in situ hybridization / Jane Bayani and Jeremy A. Squire. Quantitative trait locus mapping to identify genes for complex traits in mice / Jonathan D. Smith. cDNA microarrays / Phillip G. Febbo. Mapping techniques / Simon G. Gregory. Single nucleotide polymorphisms / Nameeta Shah. Gene/protein sequence analysis : a compilation of bioinformatic tools / Bernd H.A. Rehm and Frank Reinecke.
- Protein and cell methods: Protein electrophoresis / David Sheehan and Siobhan O'Sullivan. Protein blotting / Patricia Gravel. Capillary electrophoresis of proteins / Mark Strege. Autoradiograph and fluorography / Bronwen M. Harvey. Mass spectrometry of proteins and peptides / Kenneth G. Standing. Post-translational modifications of proteins / Christoph Kannich and Birte Fuchs. Protein microarray technology / Charlotte H. Clarke and Eric T. Fung. Protein-protein interactions / Hae Ryoun Park [and others]. Glycoprotein analysis / Terry D. Butters and David C.A. Neville. Solid-phase peptide synthesis / Mare Cudic and Gregg B. Fields. Monoclonal antibodies / Zhong J. Zhang and Maher Albitar. Antibody phage display / Rob Aitken. Protein engineering / Thomas Willemsen [and others]. Directed protein evolution / Sabine C. Stebel [and others]. Enzyme linked immunosorbent assay (ELISA) / John Crowther. Epitope mapping : identification of antibody-binding sites on protein antigens / Glenn E. Morris. Quantum dots / Charles Z. Hotz. Ion-exchange chromatography / David Sheehan. Size-exclusion chromatography / Paul Cutler. Hydrophobic interaction chromatography / Paul A. O'Farrell. Affinity chromatography / Adam Charlton and Michael Zachariou. High performance liquid chromatography (HPLC) of peptides and proteins / Tzong-Hsien Lee and Marie-Isabel Aguilar. Amino acid analysis / Ian Davidson and Paula O'Connor. Surface plasmon resonance / K. Scott Phillips and Quan Jason Cheng. Macromolecular crystallography / Bernhard Rupp and Katherine A. Kantardjieff. Microchip devices for bioanalysis / Anna C. Kinsella and Shelley D. Minteer. Mammalian cell culture / Simon P. Langdon. Plant tissue culture / Victor M. Loyola-Vargas [and others]. Stem cells and regenerative medicine / Mohan C. Vemuri and Chellu S. Chetty. Cryopreservatioin : conservation of bioresources at ultra low temperatures / John G. Day [and others]. Magnetic resonance imaging / Pottumarthi V. Prasad and Pippa Storey. Electron microscopy / John Kuo. Confocal microscopy / Guy Cox. Laser microdissection / Graeme I. Murray. Biomedical uses of flow cytometry / James L. Weaver and Maryalice Stetler-Stevenson. Immunomicroscopy / Constance Oliver. In situ hybridization / Ian A. Darby and Tim D. Hewitson. High throughput screening / William P. Janzen.