Plant embryogenesis : methods and protocols /

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Bibliographic Details
Edition:	Revised.
Imprint:	New York, NY : Humana Press, [2020]
Description:	1 online resource (1 PDF file (xi, 298 pages)) : illustrations (some color)
Language:	English
Series:	Methods in molecular biology, 1940-6029 ; volume 2122 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 2122. Springer protocols (Series)
Subject:	Plant embryology -- Laboratory manuals. Botany. Embryology. Plants -- embryology. Plant Somatic Embryogenesis Techniques. Embryonic Development. Botany. Embryology. Plant embryology. Laboratory Manual. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/12040087

Hidden Bibliographic Details
Other authors / contributors:	Bayer, Martin (Plant geneticist), editor.
ISBN:	9781071603420 1071603426 1071603418 9781071603413 9781071603413 1071603418
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. Online resource; title from PDF title page (viewed February 4, 2021).
Summary:	This volume details state-of-the-art methods for the study of plant embryogenesis in the model organism Arabidopsis thaliana, other models, and non-model species. Chapters guide readers through genetic screens, phenotypic analysis, live imaging, transcriptional profiling, methods on other model and non-model species beyond Arabidopsis thaliana, and introduction to systems that allow to culture or produce zygotic and somatic embryos in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Embryogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Other form:	Printed edition 9781071603413 Printed edition 9781071603437 Printed edition 9781071603444
Standard no.:	9781071603413 10.1007/978-1-0716-0342-0

Table of Contents:

Genetic Screens to Target Embryo and Endosperm Pathways in Arabidopsis and Maize
EMS Mutagenesis of Arabidopsis Seeds
EMS Mutagenesis of Maize Pollen
Live-cell Imaging of Zygotic Intracellular Structures and Early Embryo Pattern Formation in Arabidopsis thaliana
Analyzing Sub-Cellular Reorganization during Early Arabidopsis Embryogenesis using Fluorescent Markers
From Stained Plant Tissues to Quantitative Cell Segmentation Analysis with MorphoGraphX
Small RNA In Situ Hybridizations on Sections of Arabidopsis Embryos
Manual Isolation of Living Early Embryos from Tobacco Seeds
Profiling Transcriptomes of Manually Dissected Arabidopsis Embryos
Laser-assisted Microdissection of Plant Embryos for Transcriptional Profiling
Cell-type-specific Transcriptomics in the Plant Embryo Using an Adapted INTACT Protocol
Transcriptomic Profiling of the Arabidopsis Embryonic Epidermis Using FANS in Combination with RNAseq
Visualizing Protein Associations in living Arabidopsis Embryo
Imaging of Embryo Sac and Early Seed Development in Maize after Feulgen Staining
Using Giant Scarlet Runner Bean (Phaseolus coccineus) Embryos to Dissect the Early Events in Plant Embryogenesis
Microscopical Detection of Cell Death Processes during Scots Pine Zygotic Embryogenesis
Regulation of Somatic Embryo Development in Norway Spruce
In vitro Production of Zygotes by Electro-Fusion of Rice Gametes
Isolated Microspore Culture in Brassica napus
Anther Culture in Eggplant (Solanum melongena L.).

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