Expressed protein ligation : methods and protocols /

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Bibliographic Details
Imprint:	New York, NY : Humana Press, [2020]
Description:	1 online resource (x, 362 pages) : illustrations (some color).
Language:	English
Series:	Methods in molecular biology, 1940-6029 ; 2133 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 2133. Springer protocols (Series)
Subject:	Proteins. Proteins. Protein Splicing. Chemistry Techniques, Synthetic -- methods. Proteins. Laboratory Manual.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/12284112

Hidden Bibliographic Details
Other authors / contributors:	Vila-Perelló, Miquel., editor.
ISBN:	9781071604342 1071604341 9781071604335
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. Online resource; title from PDF title page (SpringerLink, viewed April 13, 2021).
Summary:	This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.
Other form:	Printed edition 9781071604335 Printed edition 9781071604359 Printed edition 9781071604366
Standard no.:	10.1007/978-1-0716-0434-2

Table of Contents:

Methods and Applications of Expressed Protein Ligation
Protein Splicing: From the Foundations to the Development of Biotechnological Applications
Identification, Characterization, and Optimization of Split Inteins
Methods to Study the Structure and Catalytic Activity of cis-splicing Inteins
Expressed Protein Ligation: General Experimental Protocols
Peptide Hydrazides as Thioester Equivalents for the Chemical Synthesis of Proteins
Native Chemical Ligation Via N-Acylurea Thioester Surrogates Obtained by Fmoc Solid Phase Peptide Synthesis.-Fused Split Inteins: Tools for Introducing Multiple Protein Modifications
Streamlined Expressed Protein Ligation: Site-specific Antibody-drug Conjugate
In vivo Histone Labeling Using Ultrafast trans-Splicing Inteins
Site-Directed Modification of Yeast-Produced Proteins Using Expressed Protein Ligation
Site-specific Phosphorylation of PDZ Domains
Semisynthesis and Reconstitution of Nucleosomes Carrying Asymmetric Histone Modifications
Facile Semisynthesis of Ubiquitylated Peptides with the Ligation Auxiliary 2-Aminooxyethanethiol
Investigating the Effects of O-GlcNAc Modifications in Parkinson's Disease using Semi-synthetic [alpha]-synuclein
Recombinant Expression of Cyclotides Using Expressed Protein Ligation
Highly Precise Protein Semisynthesis through Ligation-Desulfurization Chemistry in Combination with Phenacyl Protection of Native Cysteines. .

Expressed protein ligation : methods and protocols /

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