Correlative light and electron microscopy III /

Correlative light and electron microscopy III /

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Bibliographic Details
Imprint:	Amsterdam, Netherlands ; Boston, Mass. : Elsevier / Academic Press, [2017] ©2017
Description:	1 online resource (xvii, 352 pages) : illustrations (some color)
Language:	English
Series:	Methods in cell biology, 0091-679X ; volume 140 Methods in cell biology ; v. 140.
Subject:	Electron microscopy -- Technique. Microscopy, Electron -- methods. Light. Electron microscopy -- Technique.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/12378885

Hidden Bibliographic Details
Other authors / contributors:	Müller-Reichert, Thomas, 1962- editor. Verkade, Paul, editor.
ISBN:	9780128099766 0128099763 9780128099759 0128099755
Notes:	Includes bibliographical references. Print version record.
Summary:	The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field. Includes a brief history and overview on CLEM methods.
Other form:	Print version: Correlative light and electron microscopy. Amsterdam, Netherlands ; Boston, Mass. : Elsevier / Academic Press, ©2017 9780128099759

Description
Summary:	Correlative Light and Electron Microscopy III, Volume 140, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics discussed in this new release include Millisecond time-resolved CLEM, Super resolution LM und SEM of high-pressure frozen C. elegans, Preservation fluorescence, super res CLEM, APEX in Tissue, Corrsight mit IBIDI flowthrough chamber, Correlative Light Atomic Force Electronic Microscopy (CLAFEM), Atmospheric EM CLEM, and High-precision correlation, amongst other topics.Chapters in this ongoing series deal with different approaches for analyzing the same specimen using more than one imaging technique. The strengths and application area of each is presented, with this volume exploring the aspects of sample preparation of diverse biological systems for different CLEM approaches.- Contains contributions from experts in the field- Covered topics include targeted ultramicrotomy and high-precision correlation- Presents recent advances and currently applied correlative approaches- Gives detailed protocols allowing the application of workflows in one's own laboratory setting- Covers CLEM approaches in the context of specific applications- Aims to stimulate the use of new combinations of imaging modalities
Physical Description:	1 online resource (xvii, 352 pages) : illustrations (some color)
Bibliography:	Includes bibliographical references.
ISBN:	9780128099766 0128099763 9780128099759 0128099755
ISSN:	0091-679X ;