CRISPR in animals and animal models /
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Imprint: | London : Academic Press, an imprint of Elsevier, 2017. |
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Description: | 1 online resource |
Language: | English |
Series: | Progress in molecular biology and translational science ; volume 152 Progress in molecular biology and translational science ; volume 152. |
Subject: | |
Format: | E-Resource Book |
URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/12378993 |
Table of Contents:
- Cover
- Title page
- Copyright
- Contents
- Contributors
- Chapter One
- CRISPR History: Discovery, Characterization, and Prosperity
- Abstract
- 1 Introduction
- 2 Discovery of CRISPR-Cas systems
- 2.1 Identification of CRISPR Loci and cas Genes
- 2.2 Prediction of Small RNA-Based Antiviral Functions of CRISPR
- 2.2.1 Identification of CRISPR-Related Small RNAs
- 2.2.2 Origin of Spacers in CRISPR Loci
- 2.3 Experimental Demonstration of the Basic Mechanism of CRISPR-Cas System
- 2.3.1 Adaptation
- 2.3.2 crRNA Biogenesis
- 2.3.3 Interference3 Molecular mechanisms of nucleic acid interference
- 3.1 PAM-Dependent DNA Interference by Type I CRISPR-Cas Systems
- 3.2 Type II CRISPR-Cas System Requires a Unique tracrRNA for DNA Interference
- 3.3 RNA-Activated DNA Interference by Type III CRISPR-Cas Systems
- 4 Discovery of anti-CRISPR system
- 5 Identification of novel CRISPR-Cas systems
- 6 Blooming of CRISPR technology
- References
- Chapter Two
- CRISPR/Cas9 Technology: Applications and Human Disease Modeling
- Abstract
- 1 Genome engineering tools
- 1.1 Genome Engineering Technologies1.1.1 Homologous Recombination (HR)
- 1.1.2 Double-Strand Breaks (DSBs)
- 1.1.3 NHEJ Versus HDR
- 1.1.4 Zinc Finger Nuclease (ZFN)
- 1.1.5 Transcription Activator-Like Effector Nucleases (TALENs)
- 1.1.6 CRISPR/Cas9
- 1.2 Off Target Effects
- 2 CRISPR applications
- 2.1 CRISPRi and CRISPRa: Regulation of Transcriptional Levels
- 2.2 Epigenome Edition
- 2.3 Genome Imaging
- 2.4 Large-Scale Functional Genomic Studies Using CRISPR/Cas9
- 2.5 Edition of Single-Stranded RNA
- 3 CRISPR applications in biomedical modeling3.1 Neurological Disease Models
- 3.2 Cancer Models
- 3.3 Cardiovascular Disease Models
- 3.4 Infectious Disease Models
- 3.5 Immunodeficiency Models
- 4 Concluding remarks
- References
- Chapter Three
- Dynamics of Indel Profiles Induced by Various CRISPR/Cas9 Delivery Methods
- Abstract
- 1 Introduction
- 1.1 Genome Editing and DNA Repair
- 1.2 Indel Detection Methodologies
- 1.3 IDAA for Indel Detection and gRNA Selection
- 2 Materials and methods
- 2.1 gRNAs, Plasmid Vectors and Cell Lines
- 2.2 Cas9 and gRNA Delivery Conditions2.3 Efficiency Measurements
- 3 Results
- 3.1 Rationale and Design of the Study
- 3.2 Transfection and Integration Dynamics
- 3.3 Efficiency of Generating Indels Over Time
- 3.4 Indel Profiles Over Time
- 3.5 Dynamic Effect of gRNA Stability on Indel Efficiency
- 3.6 Comparison of IDAA and TIDE Methods
- 4 Concluding remarks
- Acknowledgments
- References
- Chapter Four
- CRISPR Libraries and Screening
- Abstract
- 1 Introduction
- 2 Mouse models of cancer
- 3 CRISPR-Cas9 systems
- 4 CRISPR libraries