Enzymes in synthetic biology /
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| Edition: | First edition. |
|---|---|
| Imprint: | Cambridge, MA : Academic Press is an imprint of Elsevier, 2018. |
| Description: | 1 online resource (xviii, 439 pages) : illustrations (some color) |
| Language: | English |
| Series: | Methods in enzymology ; volume six hundred and eight Methods in enzymology ; v. 608. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/12379210 |
Table of Contents:
- Intro; Title page; Table of Contents; Copyright; Contributors; Preface; Section I: Enzyme Discovery; Chapter One: Enzyme Discovery: Enzyme Selection and Pathway Design; Abstract; 1 Introduction; 2 Enzyme Selection; 3 Pathway Design; 4 Summary and Conclusion; Acknowledgments; Section II: Enzyme Engineering Tools and Platforms; Chapter Two: Cell-Free Synthetic Biology for Pathway Prototyping; Abstract; 1 Introduction; 2 The Cell-Free Metabolic Engineering Framework; 3 S12 Lysate Preparation for Cell-Free Metabolic Engineering; 4 Mix-and-Match Cell-Free Metabolic Engineering
- 5 Cell-Free Protein Synthesis-Driven Metabolic Engineering6 Summary and Conclusions; Acknowledgments; Chapter Three: Fast and Flexible Synthesis of Combinatorial Libraries for Directed Evolution; Abstract; 1 Introduction; 2 Methods; 3 Library Ligation, Transformation, and Quality Control; 4 Summary and Conclusion; Acknowledgments; Section III: Enzyme Families: Parts and Platforms for Chemical Diversity; Chapter Four: Sesquiterpene Synthase-Catalyzed Conversion of a Farnesyl Diphosphate Analogue to a Nonnatural Terpenoid Ether; Abstract; 1 Introduction
- 2 Sesquiterpenes Production and Purification3 Enzymatic Synthesis of Cyclic Ether; 4 Conclusion; Chapter Five: In Vivo Platforms for Terpenoid Overproduction and the Generation of Chemical Diversity; Abstract; 1 Introduction; 2 In Vitro Reconstitution of the MVA Pathway and Targeted Engineering of Value-Added Terpenoids; 3 Genome Mining of Terpene Cyclases and the Generation of Chemical Diversity; 4 Conclusion; Acknowledgments; Chapter Six: Imine Reductases, Reductive Aminases, and Amine Oxidases for the Synthesis of Chiral Amines: Discovery, Characterization, and Synthetic Applications
- Abstract1 Introduction; 2 Protein Engineering; 3 Biotransformations, Intensification, and Scale-Up; 4 In Vitro and in vivo Cascades; 5 Conclusions; Chapter Seven: Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins; Abstract; 1 Introduction; 2 Single Mutants Generation; 3 Combining Mutations; 4 Summary and Conclusions; Acknowledgments; Chapter Eight: Characterization of Cytochrome P450 Enzymes and Their Applications in Synthetic Biology; Abstract; 1 Introduction; 2 Expression and Purification of Microbial Cytochrome P450 Enzymes
- 3 Expression and Purification of Eukaryotic P450s in E. coli4 Expression and Characterization of P450 Redox Partner Systems; 5 Determination of P450 Purity, Concentration, and Other Properties Using UV-Visible Spectroscopy; 6 Using Cytochromes P450 for Oxidative Transformations; 7 Crystallization and Structural Analysis of P450S; 8 Applications of P450 Enzymes in Synthetic Biology; 9 Summary and Conclusion; Section IV: Genome Engineering for Enzyme Pathways