CRISPR-Cas Enzymes /
Saved in:
| Edition: | First edition. |
|---|---|
| Imprint: | Cambridge, MA : Academic Press, 2019. |
| Description: | 1 online resource |
| Language: | English |
| Series: | Methods in enzymology, 0076-6879 ; volume 616 Methods in enzymology ; v. 616. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/12379316 |
Table of Contents:
- Front Cover; CRISPR-Cas Enzymes; Copyright; Contents; Contributors; Preface; Chapter One: Predicting and visualizing features of CRISPR-Cas systems; 1. Introduction; 2. Identify and characterize CRISPR-Cas system enzymes; 2.1. Requirements; 2.2. Metagenomic preparation; 2.3. Annotation; 2.4. CRISPR-Cas system identification and typing; 3. Visualize and characterize the repeat-spacer array; 3.1. Requirements; 3.2. Extract and visualize the repeat spacer array; 3.3. Determine CRISPR locus orientation; 4. crRNA guides; 4.1. Requirements; 4.2. tracrRNA identification
- 4.3. crRNA boundaries and tracrRNA identification through RNASeq5. Predicting the PAM sequence; 5.1. Requirements; 5.2. Procedure; Acknowledgments; References; Chapter Two: Reconstitution and biochemical characterization of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems; 1. Introduction; 1.1. In vivo assay for Cascade- and Cas3-mediated target plasmid loss; 1.2. Cloning, expression, and purification of the T. fusca Cascade complex; 2. Electrophoretic mobility shift assay; 2.1. Chemical probing
- 2.2. Assemble TfuCascade/seed-bubble, TfuCascade/R-loop, and TfuCascade/R-loop/Cas3 complexes for structural analysis3. Conclusion; Acknowledgments; References; Further reading; Chapter Three: Sortase-mediated fluorescent labeling of CRISPR complexes; 1. Introduction; 2. Methods; 2.1. Overview; 2.2. Buffers; 2.2.1. Sortase purification buffers; 2.2.2. Cas1-Cas2 purification buffers; 2.2.3. Cascade purification buffers; 2.2.4. Cas3 purification buffers; 2.3. Sortase purification; 2.4. Purification of Tfu Cascade complexes and subunits for N-terminal sortase labeling
- 2.4.1. Cas1-Cas2 purification and sortase labeling of Cas22.4.2. Cascade purification and sortase labeling of Cse1; 2.5. Purification of Tfu Cascade complexes and subunits for C-terminal sortase labeling; 2.5.1. Cas3 purification and sortase labeling; 2.6. Optimization of sortase-mediated fluorescent labeling; 3. Applications; 3.1. Single-molecule imaging of fluorescent Cas1-Cas2, Cascade, and Cas3 on DNA curtains; 4. Notes; Acknowledgments; Funding; References; Chapter Four: Fluorescence-based methods for measuring target interference by CRISPR-Cas systems; 1. Introduction
- 2. Fluorescence-based strategies for measuring CRISPR interference2.1. Design and development of GFP-reporter plasmid pACYC-GFP; 2.2. Validation of GFP-based plasmid-loss assay; 3. Measurement of CRISPR interference in colonies and liquid culture; 3.1. Addition of CRISPR target to pACYC-GFP; 3.1.1. Equipment; 3.1.2. Buffers and reagents; 3.1.3. Procedure; 3.2. Detection of plasmid levels in bacterial colonies; 3.2.1. Equipment; 3.2.2. Buffers and reagents; 3.2.3. Procedure; 3.3. Measurement of CRISPR interference efficiency in liquid cultures; 3.3.1. Equipment; 3.3.2. Buffers and reagents