Cell-derived matrices. Part A /
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| Imprint: | Cambridge : Academic Press, 2020. |
|---|---|
| Description: | 1 online resource |
| Language: | English |
| Series: | Methods in cell biology ; v. 156 Methods in cell biology ; v. 156. |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/12379942 |
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| 245 | 0 | 0 | |a Cell-derived matrices. |p Part A / |c edited by David Caballero, Subhas C. Kundu, Rui Luís Reis. |
| 260 | |a Cambridge : |b Academic Press, |c 2020. | ||
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| 490 | 1 | |a Methods in cell biology ; |v v. 156 | |
| 505 | 0 | |a Front Cover -- Cell-Derived Matrices -- Part A -- Copyright -- Contents -- Contributors -- Preface -- Section I: Decellularization and characterization methods -- Chapter 1: A step-by-step protocol for generating human fibroblast cell-derived completely biological extracellular matrix ... -- 1. Introduction -- 2. Materials and methods -- 2.1. PDMS substrate fabrication -- 2.2. Collagen grafting on PDMS substrates -- 2.3. Human dermal fibroblast sheet culture and decellularization procedure -- 3. Evaluation of hDF derived ECM scaffold -- 3.1. Immunofluorescence imaging | |
| 505 | 8 | |a 3.2. Field emission scanning electron micrography -- 4. Discussion -- 5. Conclusion -- Acknowledgments -- References -- Chapter 2: Preparation of osteogenic matrices from cultured cells -- 1. Introduction -- 2. Osteogenic tissue culture and relevant assays -- 2.1. Human MSC expansion and osteogenic differentiation -- 2.1.1. Materials -- 2.1.2. Procedure -- 2.2. Assays of OPG secretion by enzyme linked immunosorbent assay (ELISA) -- 2.2.1. Materials -- 2.2.2. Procedure -- 2.3. Assays of ALP activity -- 2.3.1. Materials -- 2.3.2. Procedure -- 2.4. Mineralization assays -- 2.4.1. Materials | |
| 505 | 8 | |a 2.4.2. Procedure -- 2.5. Quantitative real-time RT-PCR (qRT-PCR) assays of biomarker transcription -- 3. Purification and characterization of OECM -- 3.1. Purification of matrix -- 3.1.1. Materials -- 3.1.2. Procedure -- 3.2. Assays of protein and calcium -- 3.2.1. Materials -- 3.2.2. Procedures -- 3.3. Morphology of OECM -- 3.4. Composition of OECM -- 3.4.1. Materials -- 3.4.2. Procedure -- 4. Coating of OECM onto growth surfaces -- 4.1. Solubilization and coating of OECM onto tissue culture plastic -- 4.1.1. Materials -- 4.1.2. Procedure | |
| 505 | 8 | |a 4.2. In situ coating of OECM by culture and decellularization -- 4.2.1. Materials -- 4.2.2. Procedure -- 5. Discussion, future prospects -- Acknowledgments -- References -- Chapter 3: Decellularized hASCs-derived matrices as biomaterials for 3D in vitro approaches -- 1. Introduction -- 2. Materials and methods -- 2.1. Reagents, equipment and material -- 2.2. CELL-derived ECM production -- 2.3. CELL-derived ECM production -- 3. Evaluation of effectiveness of the decellularization -- 3.1. Reagents, equipment and material -- 3.2. DNA quantification -- 3.3. Visualization of nuclear material | |
| 505 | 8 | |a 3.4. Qualitative evaluation of collagen -- 4. Discussion -- 5. Conclusions -- Acknowledgments -- References -- Chapter 4: Characterization of the elastic properties of extracellular matrix models by atomic force microscopy -- 1. Introduction -- 2. Material, equipments and reagents -- 2.1. Equipment -- 2.1.1. AFM -- 2.1.2. Optical microscope -- 2.1.3. Specific equipment for sample preparation -- 2.2. Material and reagents -- 2.2.1. Measurement liquid buffer -- 2.2.2. AFM cantilever-tip -- 3. Protocol to measure the mechanical properties of ECM models | |
| 650 | 0 | |a Extracellular matrix. |0 http://id.loc.gov/authorities/subjects/sh85057464 | |
| 650 | 7 | |a Extracellular matrix. |2 fast |0 (OCoLC)fst00918995 | |
| 655 | 4 | |a Electronic books. | |
| 700 | 1 | |a Caballero, David. | |
| 700 | 1 | |a Kundu, S. C. |q (Subhas Chandra) |0 http://id.loc.gov/authorities/names/nb2014010807 |1 http://viaf.org/viaf/308759548 | |
| 700 | 1 | |a Reis, Rui L. |0 http://id.loc.gov/authorities/names/n2002159105 |1 http://viaf.org/viaf/44591901 | |
| 776 | 0 | 8 | |i Print version: |z 012820172X |z 9780128201725 |w (OCoLC)1119625472 |
| 830 | 0 | |a Methods in cell biology ; |v v. 156. |0 http://id.loc.gov/authorities/names/n91115463 | |
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