RNA remodeling proteins : methods and protocols /

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Bibliographic Details
Edition:	Second edition.
Imprint:	New York : Humana Press ; Springer, [2021].
Description:	1 online resource
Language:	English
Series:	Methods in molecular biology, 1064-3745 ; 2209 Methods in molecular biology (Clifton, N.J.) ; 2209.
Subject:	RNA -- Laboratory manuals. Proteins -- Laboratory manuals. Proteins. RNA. Proteins. RNA. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/12451824

Hidden Bibliographic Details
Other authors / contributors:	Boudvillain, Marc, editor.
ISBN:	9781071609354 1071609351 1071609343 9781071609347
Notes:	Includes bibliographical references and index (pages 433-437).
Summary:	This volume provides the most current methods to study RNA remodeling proteins. Chapters detail methods, ranging from basic to complex, procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex molecular mechanisms of action using purified components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Remodeling Proteins: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Other form:	Print version : RNA remodeling proteins: methods and protocols 1071609343 9781071609347
Standard no.:	10.1007/978-1-0716-0935-4

Table of Contents:

Key Points to Consider when Studying RNA Remodeling by Proteins
Happy Birthday : 30 Years of RNA Helicases
Known Inhibitors of RNA Helicases and their Therapeutic Potential
Identifying RNA Helicase Inhibitors Using Duplex Unwinding Assays
Thermal Shift Assay for Characterizing the Stability of RNA Helicases and their Interaction with Ligands
SRCD and FTIR Spectroscopies to Monitor Protein-induced Nucleic Acid Remodeling
High throughput Protein-Nucleic Acid Interaction Assay Based on Protein Induced Fluorescence Enhancement
Probing RNA Helicase Conformational Changes by Single-molecule FRET Microscopy
A Fluorescent Assay to Monitor Ligand-Dependent Closure of the Hexameric Rho Helicase Ring
A Simple Fluorescence Microplate Assay to Monitor RNA-DNA Hybrid Unwinding by the Bacterial Transcription Termination Factor Rho
Monitoring Enzymatic RNA G-quadruplex Unwinding Activities by Nuclease Sensitivity and Reverse Transcription Stop Assays
Using Magnetic Tweezers to Unravel the Mechanism of the G-quadruplex Binding and Unwinding Activities of DHX36 Helicase
Characterization of the Brr2 RNA Helicase and its Regulation by other Spliceosomal Proteins using Gel-based U4/U6 di-snRNA Binding and Unwinding Assays
Monitoring Acetylation of the RNA-helicase DDX3X, a Protein Critical for Formation of Stress Granules
In vivo cross-linking Followed by polyA Enrichment to Identify Yeast mRNA Binding Proteins
Deciphering the Dynamic Landscape of Transcription-associated mRNP quality Control Components over the Whole Yeast Genome
Probing the Conformational State of mRNPs using smFISH and SIM
Probing Transcriptome-wide RNA Structural Changes Dependent on the DEAD-box Helicase Dbp2
In situ hybridization Proximity Ligation Assay (ISH-PLA) to Study the Interaction of HIV-1 RNA and Remodeling Proteins
RNAi Screening to Identify Factors that Control Circular RNA Localization
A Cell-Free System for Investigating Human MARF1 Endonuclease Activity
Monitoring eIF4E-dependent Nuclear 3 end mRNA Cleavage
An In Vitro Approach To Study RNase III Activities Of Plant RTL Proteins
Analysis of Bacillus subtilisRibonuclease Activity In Vivo
Assay of Bacillus subtilis Ribonuclease Activity in vitro
Protein Pulldown Assays to Monitor the Composition of the Bacterial RNA Degradosome. .

RNA remodeling proteins : methods and protocols /

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