Evaluation of Cellular Processes by in Vitro Assays /
Saved in:
| Author / Creator: | Gul, Taseen. |
|---|---|
| Imprint: | Sharjah : Bentham Science Publishers, 2018. |
| Description: | 1 online resource (106 pages) |
| Language: | English |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/12484252 |
Table of Contents:
- Intro; CONTENTS; FOREWORD; PREFACE; ABBREVIATIONS; ABBREVIATIONS; Basics of In Vitro Cell Culture; INTRODUCTION; 1.1. Tissue Culture and Its Types; 1.2. Cell Line; 1.3. Types of Culture; 1.4. Equipments for Cell Culture Lab; 1.5. Materials for Cell Culture; 1.6. Cryopreservation of Cell Lines; 1.7. Cell Line Authentication; 1.8. Contamination in Cell Lines; CONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Methods of Transfection; INTRODUCTION; 2.1. Chemical Methods of Transfection; 2.2. Physical Methods of Gene Delivery.
- 2.3. Biological Methods of TransfectionCONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Apoptosis; INTRODUCTION; 3.1. Mediators of Apoptosis; 3.2. Activation of Apoptosis; 3.3. Methods for Detecting Apoptosis; 3.3.1. Analysing Cytomorphological Alterations; 3.3.2. DNA Fragmentation for Detecting Apoptosis; 3.3.3. Mitochondrial Markers for Detecting Apoptosis; 3.3.4. Measuring Changes in Cell Membrane to Detect Apoptosis; 3.3.5. Measuring Caspase Activity for the Detection of Apoptosis; CONCLUDING REMARKS; CONSENT FOR PUBLICATION.
- CONFLICT OF INTERESTACKNOWLEDGEMENTS; REFERENCES; Cell Cytotoxicity, Viability and Proliferation; INTRODUCTION; 4.1. Trypan Blue Assay; 4.2. Neutral Red (3-Amino-7-Dimethyamino-2-Methylphenazine Hydrochlo- ride); 4.3. Calcein AM; 4.4. CFSE; 4.5. Sulphorhodamine B Assay; 4.6. Assays Based on Metabolic Activity; 4.7. Lactate Dehydrogenase Assay; 4.8. ATP Bioluminescent Assays; 4.9. Clonogenic Assay; 4.10. DNA Synthesis as a Marker for Cell Proliferation; CONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Reactive Oxygen Species (ROS); INTRODUCTION.
- 5.1. Detection of Lipid Peroxidation5.2. Glutathione Assay; 5.3. Superoxide Assay; 5.4. Nitric Oxide Detection Assay; 5.5. Hydrogen Peroxide Detection Assay; CONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Protein-Protein Interactions; INTRODUCTION; 6.1. Co-Immunoprecipitation; 6.2. Pull Down Assays; 6.3. Far-Western Blotting; CONCLUSION; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; Protein-Nucleic Acid Interactions; INTRODUCTION; 7.1. Electrophoretic Mobility Shift Assay (EMSA).
- 7.2. Chromatin Immunoprecipitation (ChIP) Assay7.3. Affinities Capture Methods; 7.4. Pull-Down Methods; CONCLUDING REMARKS; CONSENT FOR PUBLICATION; CONFLICT OF INTEREST; ACKNOWLEDGEMENTS; REFERENCES; GLOSSARY; SUBJECT INDEX.