Programmed morphogenesis : methods and protocols /

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Bibliographic Details
Imprint:New York, NY : Humana Press, 2021.
Description:1 online resource
Language:English
Series:Methods in Molecular Biology, 1064-3745
Methods in molecular biology (Clifton, N.J.)
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/12489428
Hidden Bibliographic Details
Other authors / contributors:Ebrahimkhani, Mo R.
Hislop, Joshua.
ISBN:9781071611746
1071611747
1071611739
9781071611739
Digital file characteristics:text file
PDF
Notes:Includes index.
Online resource; title from PDF title page (SpringerLink, viewed February 15, 2021).
Summary:This detailed book explores techniques for understanding and engineering programs that naturally control and drive formation of tissues and organs in order to open powerful opportunities to produce physiologically relevant tissues of interest, generate models to study human disease, and set the path for the manufacturing of advanced tissue and organs. Beginning with chapters to help understand signaling events and patterns in morphogenetic systems, the volume continues by covering programming signaling events and patterns to drive morphogenesis, techniques for engineering organoids, tissue barriers, and disease models, as well as in vivo therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Programmed Morphogenesis: Methods and Protocols aims not only to communicate knowledge but also to inspire approaches to new challenges and to empower readers with the capability to approach those challenges.
Other form:Print version: Programmed morphogenesis. New York, NY : Humana Press, 2021 1071611739 9781071611739
Standard no.:10.1007/978-1-0716-1174-6

MARC

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505 0 |a TASBE Image Analytics: A Processing Pipeline for Quantifying Cell Organization from Fluorescent Microscopy -- Neighborhood Impact Factor to Study Cell-Fate Decision-Making in Cellular Communities -- A Quantitative Lineage-Tracing Approach to Understand Morphogenesis in Gut -- Reconstitution of Morphogen Signaling Gradients in Cultured Cells -- Engineering Shape-Controlled Microtissues on Compliant Hydrogels with Tunable Rigidity and Extracellular Matrix Ligands -- Engineering Biophysical Cues for Controlled 3D Differentiation of Endoderm Derivatives -- Rewiring Endogenous Bioelectric Circuits in the Xenopus laevis Embryo Model -- Engineering the Spatiotemporal Mosaic Self-Patterning of Pluripotent Stem Cells -- Fate-Patterning of 2D Gastruloids and Ectodermal Colonies Using Micropatterned Human Pluripotent Stem Cells -- Gastruloids: Embryonic Organoids from Mouse Embryonic Stem Cells to Study Patterning and Development in Early Mammalian Embryos -- A Synergistic Engineering Approach to Build Human Brain Spheroids -- Directed Differentiation of Human Pluripotent Stem Cells for the Generation of High-Order Kidney Organoids -- Methods for Controlled Induction of Singular Rosette Cytoarchitecture within Human Pluripotent Stem Cell-Derived Neural Multicellular Assemblies -- 3D Self-Organized Human Blood-Brain Barrier in a Microfluidic Chip -- Modeling the Complexity of the Metastatic Niche Ex Vivo -- Fabrication Method of a High-Density Co-Culture Tumor-Stroma Platform to Study Cancer Progression -- A Method for Organoid Transplantation and Whole-Mount Visualization of Post-Engraftment Vascularization -- High Throughput Production of Platelet-Like Particles. 
500 |a Includes index. 
520 |a This detailed book explores techniques for understanding and engineering programs that naturally control and drive formation of tissues and organs in order to open powerful opportunities to produce physiologically relevant tissues of interest, generate models to study human disease, and set the path for the manufacturing of advanced tissue and organs. Beginning with chapters to help understand signaling events and patterns in morphogenetic systems, the volume continues by covering programming signaling events and patterns to drive morphogenesis, techniques for engineering organoids, tissue barriers, and disease models, as well as in vivo therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Programmed Morphogenesis: Methods and Protocols aims not only to communicate knowledge but also to inspire approaches to new challenges and to empower readers with the capability to approach those challenges. 
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