Structural proteomics : high-throughput methods /

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Bibliographic Details
Edition:	Third edition.
Imprint:	New York, NY : Humana Press, [2021]
Description:	1 online resource (xii, 344 pages) : illustrations (some color).
Language:	English
Series:	Methods in molecular biology, 1064-3745 Methods in molecular biology (Clifton, N.J.),
Subject:	Proteomics -- Laboratory manuals. Proteins -- Structure -- Laboratory manuals. Proteins -- Structure. Proteomics. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/12574398

Hidden Bibliographic Details
Other authors / contributors:	Owens, Raymond J., editor.
ISBN:	9781071614068 1071614061 9781071614051
Notes:	Includes index. Online resource; title from PDF title page (SpringerLink, viewed May 11, 2021).
Summary:	This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins. Beginning with a section on structural bioinformatics, the book continues with sections covering the challenge of producing high quality samples for structural studies, particularly mammalian membrane proteins and protein complexes, as well as protocols for structure determination, including the use of electrons in structural biology and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Structural Proteomics: High-Throughput Methods, Third Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science. Chapter 9 is available open access under a CC BY 4.0 license.
Standard no.:	10.1007/978-1-0716-1406-8

Table of Contents:

The Protein Data Bank Archive
Computational Methods for the Elucidation of Protein Structure and Interactions
Methods for Molecular Modeling of Protein Complexes
High-Level Production of Recombinant Eukaryotic Proteins from Mammalian Cells Using Lentivirus
Transient Transfection and Expression of Eukaryotic Membrane Proteins in Expi293F Cells and Their Screening on a Small-Scale: Application for Structural Studies
Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells
SynBac: Enhanced Baculovirus Genomes by Iterative Recombineering
Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification
Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems
Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins
Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources
From Tube to Structure: SPA Cryo-EM Workflow Using Apoferritin as an Example
Image Processing in Cryo-Electron Microscopy of Single Particles: The Power of Combining Methods
Setup and Troubleshooting of Volta Phase Plate Cryo-EM Data Collection
Cryo-Focused Ion Beam Lamella Preparation Protocol for In Situ Structural Biology
Protein and Small Molecule Structure Determination by the Cryo-EM Method MicroED.

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