Fluorescence in situ hybridization (FISH) : application guide /
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| Imprint: | Berlin ; London : Springer, ©2009. |
|---|---|
| Description: | 1 online resource (xviii, 451 pages) : illustrations (some color) |
| Language: | English |
| Series: | Springer protocols Springer protocols (Series) |
| Subject: | |
| Format: | E-Resource Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/12587120 |
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| 245 | 0 | 0 | |a Fluorescence in situ hybridization (FISH) : |b application guide / |c Thomas Liehr, editor. |
| 260 | |a Berlin ; |a London : |b Springer, |c ©2009. | ||
| 300 | |a 1 online resource (xviii, 451 pages) : |b illustrations (some color) | ||
| 336 | |a text |b txt |2 rdacontent | ||
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| 338 | |a online resource |b cr |2 rdacarrier | ||
| 347 | |a text file | ||
| 347 | |b PDF | ||
| 490 | 1 | |a Springer protocols | |
| 504 | |a Includes bibliographical references and index. | ||
| 588 | 0 | |a Print version record. | |
| 520 | |a This FISH application guide provides an overview of the principles and the basic techniques of fluorescence in situ hybridization (FISH) and primed in situ hybridization (PRINS), which are successfully used to study many aspects of genomic behavior and alterations. In 36 chapters, contributed by international experts in their particular field, the nowadays multiple approaches and applications of the powerful techniques are presented and detailed protocols are given. Described here are methods using various cell types and tissues as well as different organisms, such as mammalians, insects, plants and microorganisms. Multicolor FISH procedures and special applications such as the characterization marker chromosomes, breakpoints, cryptic aberrations, nuclear architecture and epigenetic changes, as well as (array-based) comparative genomic hybridization studies are presented. Overall, the technique of choice is introduced for single cell analysis in human genetics, microbiology, animal and plant sciences. | ||
| 505 | 0 | |a Molecular Cytogenetic Applications in Diagnostics and Research: An Overview; Molecular Cytogenetics: The Standard FISH and PRINS Procedure; Generation of Paint Probes by Flow-Sorted and Microdissected Chromosomes; ISH Probes Derived from BACs, Including Microwave Treatment for Better FISH Results; PNA-FISH Technique for In Situ Assessment of Aneuploidy; Oligonucleotide FISH Probes; Microscopy and Imaging Systems; Optical Filters for FISH; FISH With and Without COT1 DNA; Pre- and Postnatal Diagnostics and Research on Peripheral Blood, Chorion, Amniocytes, and Fibroblasts. | |
| 546 | |a English. | ||
| 650 | 0 | |a Fluorescence in situ hybridization. |0 http://id.loc.gov/authorities/subjects/sh98005669 | |
| 650 | 2 | |a Situ Hybridization, Fluorescence |x methods | |
| 650 | 6 | |a Hybridation in situ fluorescente. | |
| 650 | 7 | |a SCIENCE |x Life Sciences |x Molecular Biology. |2 bisacsh | |
| 650 | 0 | 7 | |a Fluorescence in situ hybridization. |2 cct |
| 650 | 7 | |a Sciences de la vie. |2 eclas | |
| 650 | 7 | |a Biomédecine. |2 eclas | |
| 650 | 7 | |a Fluorescence in situ hybridization. |2 fast |0 (OCoLC)fst00928101 | |
| 700 | 1 | |a Liehr, Thomas, |d 1965- |0 http://id.loc.gov/authorities/names/n2001017520 | |
| 776 | 0 | 8 | |i Print version: |t Fluorescence in situ hybridization (FISH). |d Berlin ; London : Springer, ©2009 |z 9783540705802 |w (DLC) 2008930215 |w (OCoLC)288984856 |
| 830 | 0 | |a Springer protocols (Series) | |
| 856 | 4 | 0 | |u https://link.springer.com/10.1007/978-3-540-70581-9 |y Springer Nature |
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| 928 | |t Library of Congress classification |a RB43.8.F58F58 2009 |l Online |c UC-FullText |u https://link.springer.com/10.1007/978-3-540-70581-9 |z Springer Nature |g ebooks |i 12578609 | ||