Bacterial persistence : methods and protocols /

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Bibliographic Details
Edition:	Second edition.
Imprint:	New York, NY : Humana Press, 2021.
Description:	1 online resource (xi, 292 pages) : illustrations (some color).
Language:	English
Series:	Methods in molecular biology, 1940-6029 Methods in molecular biology (Clifton, N.J.),
Subject:	Bacteria -- Physiology -- Laboratory manuals. Drug resistance -- Laboratory manuals. Bacteriology -- Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/12660405

Hidden Bibliographic Details
Other authors / contributors:	Verstraeten, Natalie, editor. Michiels, Jan (Professor), editor.
ISBN:	9781071616215 1071616218 9781071616208 107161620X
Notes:	Includes index. Online resource; title from PDF title page (SpringerLink, viewed October 5, 2021).
Summary:	This volume presents a collection of methods that have contributed to the current understanding of bacterial persisters. Chapters in the book detail general guidelines for measuring persister levels in bacterial cultures, strategies to enrich and resuscitate persister subpopulations, single-cell approaches for visualizing and characterizing persisters, omics techniques and cellular and animal models for studying persistence. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols, Second Edition aims to be a useful practical guide to researchers to help further their study in this field.
Other form:	Original 107161620X 9781071616208
Standard no.:	10.1007/978-1-0716-1621-5

Table of Contents:

Studying Bacterial Persistence: Established Methods And Current Advance
Antibiotic Tolerance and Persistence Studied Throughout Bacterial Growth Phases
A Robust Method For Generating, Quantifying, and Testing Large Numbers of Escherichia coli persisters
Enrichment of Persister Cells through [beta]-lactam-induced Filamentation and Size Separation
Methods for Enrichment of Bacterial Persister Populations For Phenotypic Screens And Genomic Studies
Observing Bacterial Persistence at Single-Cell Resolution
Phenotypic Characterization of Antibiotic Persisters at the Single-Cell Level: From Data Acquisition to Data Analysis
Microfluidics for Single-Cell Study of Antibiotic Tolerance and Persistence Induced by Nutrient Limitation
Counting Chromosomes in Individual Bacteria to Quantify their Impacts on Persistence
Analyzing PERSISTER PROTEOMES with SILAC and Label-Free Methods
Defining Proteomic Signatures To Predict Multi-Drug Persistence in Pseudomonas aeruginosa
The use of Experimental Evolution to Study The Response of Pseudomonas aeruginosa to Single or Double Antibiotic Treatment
Detecting persister awakening determinants
Monitoring Persister Resuscitation with Flow Cytometry
Stimulating Aminoglycoside Uptake to Kill Staphylococcus aureus persisters
In vitro Models for the Study of the Intracellular Activity Of Antibiotics
Analysis of Salmonella Persister Population Sizes, Dynamics Of Gut Luminal Seeding, And Plasmid Transfer In Mouse Models Of Salmonellosis
Studying Antibiotic Persistence During Infection.

Bacterial persistence : methods and protocols /

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