Protocols in advanced genomics and allied techniques /

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Bibliographic Details
Author / Creator:	Pal, Aruna, author.
Imprint:	New York, NY : Springer, [2022]
Description:	1 online resource (1 PDF file (xxix, 720 pages) : illustrations (black and white, and color).
Language:	English
Series:	Springer protocols handbooks, 1949-2456 Springer protocols (Series)
Subject:	Genomics -- Laboratory manuals. Genomes. Genomics -- methods Genome Genomics Laboratory Manual Laboratory manuals Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/12683838

Hidden Bibliographic Details
ISBN:	9781071618189 1071618180 1071618172 9781071618172
Digital file characteristics:	text file PDF
Notes:	Includes bibliographical references and index. Description based on online resource; title from PDF title page (viewed July 12, 2022).
Summary:	This laboratory manual includes the latest tools and techniques involved in genomic research. It starts with an introductory chapter on genomics and the various tools and applications involved. The initial chapters present protocols for basic techniques such as DNA isolation, electrophoresis, PCR, cDNA synthesis etc. The book then goes on to describe more advanced techniques such as next-generation sequencing, exome sequencing, use of RNAi, RNAseq, genome editing, single cell genomics etc. Each topic includes a brief description, information on the principles involved, materials & methods, protocol, and expected results, with diagrams and graphs. All protocols are presented in a very lucid and precise way, to make it easy for readers to follow and replicate them.
Other form:	Print version: PAL, ARUNA. PROTOCOLS IN ADVANCED GENOMICS AND ALLIED TECHNIQUES. [Place of publication not identified] : SPRINGER, 2021 1071618172
Standard no.:	10.1007/978-1-0716-1818-9

Table of Contents:

Chapter 1: Conventional and Basic genomic techniques
Chapter 2: Recombinant DNA technology
Chapter 3: Mitochondrial Genetics
Chapter 4: Basic principle of Cloning
Chapter 5: Immunohistochemistry
Chapter 6: Western blotting
Chapter 7: Introduction to Bioinformatics
Chapter 8: Molecular evolutionary Study-Phylogenetic tree
Chapter 9: Real Time or Quantitative PCR
Chapter 10: Tissue or Cell Cultures
Chapter 11: DNA Micro Array
Chapter 12: Epigenetics and DNA Methylation
Chapter 13: RNA sequencing
Chapter 14: Next Generation sequencing
Chapter 15: Metagenomics
Chapter 16: Genome wide association studies (GWAS)/ SNP chips
Chapter 17: Exome sequencing
Chapter 18: RNA Interference
Chapter 19: Expressing cloned genes for protein production, purification and analysis-Biopharming
Chapter 20: Transgenesis and Biopharming
Chapter 21: Rapid amplification of cDNA ends (RACE)
Chapter 22: Gene Therapy
Chapter 23: Nutrigenomics
Chapter 24: Gene Editing
Chapter 25: Introduction to stem cell biology
Chapter 26: Transcriptomics
Chapter 27: Development of Biomarker
Chapter 28: Single cell genomics
Chapter 29: Big data Analysis.

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