Plant secondary metabolism engineering : methods and protocols /

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Bibliographic Details
Edition:Second edition.
Imprint:New York, NY : Humana Press, [2022]
Description:1 online resource (1 PDF file (xii, 262 pages)) : illustrations (some color).
Language:English
Series:Methods in molecular biology, 1940-6029 ; 2469
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 2469.
Springer protocols (Series)
Subject:
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/13436845
Hidden Bibliographic Details
Other authors / contributors:Fett-Neto, Arthur Germano, 1967- editor.
ISBN:9781071621851
1071621858
9781071621844
Notes:Includes bibliographical references and index.
Description based on online resource; title from PDF title page (SpringerLink, viewed February 7, 2023).
Summary:This second edition provides detailed practical information on important methods employed in the engineering of plant secondary metabolism pathways. New and updated chapters guide readers through extraction, quantification, purification, localization, characterization, data mining and processing, biosynthesis modulation, and pathway engineering of representative classes of plant specialized metabolites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, list of necessary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step descriptions of readily reproducible protocols. Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Protocols, Second Edition aims to be a useful practical guide to help researchers working in this exciting field.
Standard no.:10.1007/978-1-0716-2185-1

MARC

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505 0 |a Extraction and Analysis of Phenolic Compounds From Grape Berries -- Construction of the UDP-glucose biosynthetic Enzyme Gene Co-Expression Plasmid For Prunasin Production in Escherichia coli -- Histochemical Localization of Phenolic Compounds And Reactive Oxygen Species In Eucalypt Microcuttings -- A Bioinformatics Tool for Efficient Retrieval of High-Confidence Terpene Synthases (TPS) and Application to the Identification of TPS in Coffea and Quillaja -- Use of Catharanthus roseus Cell Cultures for the Synthesis Of Metal Nanoparticles -- Fungal Pigment Assisted Silver Nanoparticle Synthesis and their Antimicrobial and Cytotoxic Potential -- Nuclei Enrichment from Sugarcane Stems for Proteomics Analyses -- Visualization of Suberization and Lignification in Sugarcane -- Isolation of Promoters and Transcription Factors Involved In The Regulation of Lignin Biosynthesis in Saccharum species -- Soapbark Triterpenes: Quillaja brasiliensis Cell Culture Sapogenin and Free Sterol Analysis by GCMS -- Generation of stable Catharanthus roseus hairy root lines with Agrobacterium rhizogenes -- Analyzing the Distribution of Specialized Metabolites From Plant Native Tissues With Laser Desorption Low-Temperature Plasma Mass Spectrometry Imaging -- Virus-Induced Gene Silencing as a Tool to Study Regulation of Alkaloid Biosynthesis in Medicinal Plants -- Cyclotide isolation from Psychotria brachyceras and Psychotria leiocarpa -- Formulation of IMXQB: nanoparticles based on Quillaja brasiliensis saponins to be used as vaccine adjuvants -- Isolation of specialized plant cells by fluorescence-activated cell sorting -- X-Ray Computed Tomography for 3D Anatomical Analysis of Resin Ducts -- Untargeted metabolomic analysis of non-volatile and volatile glucosinolates in Brassicaceae -- Methods of mimosine extraction from Leucaena leucocephala (Lam.) de Wit leaves -- Microbial production, extraction, and quantitative analysis of isoprenoids. 
520 |a This second edition provides detailed practical information on important methods employed in the engineering of plant secondary metabolism pathways. New and updated chapters guide readers through extraction, quantification, purification, localization, characterization, data mining and processing, biosynthesis modulation, and pathway engineering of representative classes of plant specialized metabolites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, list of necessary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step descriptions of readily reproducible protocols. Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Protocols, Second Edition aims to be a useful practical guide to help researchers working in this exciting field. 
588 0 |a Description based on online resource; title from PDF title page (SpringerLink, viewed February 7, 2023). 
650 0 |a Biochemical engineering  |v Laboratory manuals. 
650 0 |a Plants  |x Effect of stress on  |x Research  |v Laboratory manuals. 
650 0 |a Biotransformation (Metabolism)  |x Research  |v Laboratory manuals. 
650 0 |a Metabolism, Secondary  |x Research  |v Laboratory manuals. 
650 0 |a Genetics  |x Technique.  |0 http://id.loc.gov/authorities/subjects/sh85053881 
650 1 2 |a Plants  |x metabolism  |0 https://id.nlm.nih.gov/mesh/D010944Q000378 
650 1 2 |a Metabolic Engineering  |x methods  |0 https://id.nlm.nih.gov/mesh/D060847Q000379 
650 2 2 |a Secondary Metabolism  |0 https://id.nlm.nih.gov/mesh/D064210 
650 2 |a Genetic Techniques  |0 https://id.nlm.nih.gov/mesh/D005821 
650 6 |a Génie biochimique  |v Manuels de laboratoire. 
650 6 |a Plantes  |x Effets du stress sur  |x Recherche  |v Manuels de laboratoire. 
650 6 |a Biotransformation (Métabolisme)  |x Recherche  |v Manuels de laboratoire. 
650 6 |a Métabolisme secondaire  |x Recherche  |v Manuels de laboratoire. 
650 6 |a Génétique  |x Technique. 
650 7 |a Biochemical engineering  |2 fast 
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