Applications of genome modulation and editing /

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Bibliographic Details
Imprint:	New York, NY : Humana Press, 2022.
Description:	1 online resource (xii, 369 pages) : illustrations (some color).
Language:	English
Series:	Methods in molecular biology, 1940-6029 Methods in molecular biology (Clifton, N.J.),
Subject:	Gene editing -- Laboratory manuals. Gene editing. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/13460148

Hidden Bibliographic Details
Other authors / contributors:	Verma, Paul J., editor. Sumer, Huseyin, editor. Liu, Jun, editor.
ISBN:	9781071623015 107162301X 9781071623008
Notes:	Includes index. Online resource; title from PDF title page (SpringerLink, viewed June 21, 2022).
Summary:	This volume details generation of gene-edited cell lines and organisms as models for human diseases, pest control, and large animal welfare and production outcomes. Chapters guide readers through gene regulation, editing, screening of cell lines, genome editing, and an overview of the tools for efficient genome editing including; ZFNs, TALENs, and CRISPR. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step- by-step, readily reproducible protocols. Authoritative and cutting-edge, Applications of Genome Modulation and Editing aims to be a useful and practical guide for researchers to commence or advance their study in this field.
Standard no.:	10.1007/978-1-0716-2301-5

Table of Contents:

Historical DNA manipulation Overview
Tools for efficient genome editing; ZFN, TALEN, and CRISPR
Efficient generation of stable cell lines with inducible neuronal transgene expression using the piggyBac transposon system
Modifying Bacterial Artificial Chromosomes for Extended Genome Modification
Site specific editing of the genome in Pluripotent cells
Targeting the AAVS1 site with transgene cassette by CRISPR/Cas9 with an inducible transgene cassette for the to neuronal differentiation of human pluripotent stem cells
Microinjection of zygotes for CRISPR/Cas9 mediated insertion of transgenes into the murine Rosa26 safe harbour
CRISPR-on for endogenous activation of SMARCA4 expression in bovine embryos
CRISPR/Cas mutagenesis to generate novel traits in Bactrocera tryoni for Sterile Insect Technique
CRISPR/Cas9 Genome Editing in the New World Screwworm and Australian Sheep Blowfly
Generation of gene drive mice for invasive pest population suppression
Generation of a human deafness sheep model using the CRISPR/Cas system
Targeted Gene Editing in Porcine Germ Cells
Generating a Heat Tolerance Mouse Model
Generation of pigs that produce single sex progeny
Generation of Double-muscled Sheep and Goats by CRISPR/Cas9-mediated Knockout of the Myostatin Gene
Regulatory and policy considerations around applications of genome editing in agriculture.

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