Yeast metabolic engineering : methods and protocols /

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Bibliographic Details
Edition:	Second edition.
Imprint:	New York, NY : Humana Press, [2022]
Description:	1 online resource (xi, 295 pages) : illustrations (some color).
Language:	English
Series:	Methods in molecular biology, 1940-6029 ; 2513 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 2513. Springer protocols (Series)
Subject:	Yeast -- Laboratory manuals. Yeast fungi -- Biotechnology -- Laboratory manuals. Yeasts -- metabolism Metabolic Engineering Yeast Yeast fungi -- Biotechnology Laboratory Manual Laboratory manuals Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/13480607

Hidden Bibliographic Details
Other authors / contributors:	Mapelli, Valeria, editor. Bettiga, Maurizio, editor.
ISBN:	9781071623992 1071623990 9781071623985
Notes:	Includes bibliographical references and index. Online resource; title from PDF title page (SpringerLink, viewed July 6, 2022).
Summary:	This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the "universal expression system" that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.
Standard no.:	10.1007/978-1-0716-2399-2

Table of Contents:

An Overview on Selectin Marker Genes for Transformation of Saccharomyces cerevisiae
High Efficiency Plasmid DNA Transformation in Yeast
CasPER, A CRISPR/Cas9-Based Method for Directed Evolution in Genomic loci in Saccharomyces cerevisiae
Fluorescence Activated Cell Sorting as a Tool for Recombinant Strain Screening
Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology
Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris
Competent Cell Preparation and Transformation of Pichia pastoris
CRISPR/Cas9 Tool Kit for Efficient and Targeted Insertion/Deletion Mutagenesis of the Komagataella phaffii (Pichia pastoris) Genome
Plasmid-Based Gene Knockout Strategy with Subsequent Marker Recycling in Pichia pastoris
Engineering of Promoters for Gene Expression in Pichia pastoris
Molecular Tools for Leveraging the Potential of the Acid Tolerant Yeast Zygosaccharomyces bailii as Cell Factory
Golden Gate Multi-Genes Assembly Method for Yarrowia lipolytica
A Universal Gene Expression System for Novel Yeast Species
Dynamic Feeding of Pichia pastoris
Automated Evolutionary Engineering of Yeasts
Reconstruction of Genome-Scale Metabolic Model of Hansenula polymorpha using RAVEN.

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