DNA-encoded chemical libraries : methods and protocols /

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Bibliographic Details
Imprint:	New York, NY : Humana Press, 2022.
Description:	1 online resource (x, 226 pages) : illustrations (some color).
Language:	English
Series:	Methods in molecular biology, 1940-6029 Methods in molecular biology (Clifton, N.J.),
Subject:	Combinatorial chemistry -- Laboratory manuals. DNA -- Synthesis -- Laboratory manuals. Combinatorial chemistry. DNA -- Synthesis. Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/13519430

Hidden Bibliographic Details
Other authors / contributors:	Israel, David, editor. Ding, Yun, editor.
ISBN:	9781071625453 1071625454 9781071625446
Notes:	Includes index. Online resource; title from PDF title page (SpringerLink, viewed September 13, 2022).
Summary:	This volume discusses protocols that cover synthesis, screening by selection, and analysis of DNA-encoded chemical libraries (DEL). Chapters in this book focus on methods used to practice DEL technology and include solution phase library synthesis using a variety of chemistries; DNA encoding of chemical structure; design, preparation and analysis of target proteins and tool compounds; screening of soluble protein targets by affinity selection; DEL qPCR, preparative PCR and DNA sequence analysis; computational methods used to analyze selections and choose compounds for resynthesis; and analysis of hit compounds. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, DNA-Encoded Chemical Libraries: Methods and Protocols is a valuable resource for scientists interested in DEL technology for drug discovery, and will contribute to the continued advancement in this important field.
Standard no.:	10.1007/978-1-0716-2545-3

Table of Contents:

Library Synthesis: Building Block Selection, Handling, and Tracking
Library Synthesis: Building Block Validation
Library Synthesis and Analytics
Chemical Ligation of Oligonucleotide Tags to Support Encoded Chemical Library Synthesis
On-DNA Reductive Amination and Alkylation
On-DNA-1,2,3-Triazole Formation via Click Reaction
On-DNA Suzuki-Miyaura Cross-Coupling
On-DNA Pd and Cu Promoted C-N Cross-Coupling Reactions
Palladium Promoted On-DNA Heck Reactions
Ruthenium Promoted On-DNA C-H Activation Reaction
DNA-Compatible Nitro Reduction and Synthesis of Benzimidazoles
Condensation of DNA-Conjugated Imines with Homophthalic Anhydride for the Synthesis of Isoquinolones On-DNA
DNA Encoding of Natural Products
Initiating DNA-Encoded Library Synthesis with a Hexathymidine DNA Oligonucleotide
One Bead-One Compound (OBOC) Peptidomimetic Encoded Library Synthesis via Split-and-Pool Methods
DNA Conjugates as Tool Compounds for DEL Selections
Quantitation of DNA Encoded Libraries by qPCR
Target Protein Design and Pre-Selection Analysis
DEL Selections Against a Soluble Protein Target
Selection Method of DNA-Encoded Chemical Library for Irreversible Covalent Binders
Next Generation Sequencing of DNA-Encoded Libraries
Translation of DNA Sequence to Chemical Structure in DNA Encoded Libraries
Analysis of DNA-Encoded Library Screening Data: Selection of Molecules for Synthesis
High Throughput Binder Confirmation (HTBC)
High-Throughput Binder Confirmation Using Affinity Selection Mass Spectrometry.

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