Genetically Incorporated Non-Canonical Amino Acids : Methods and Protocols /

Genetically Incorporated Non-Canonical Amino Acids : Methods and Protocols /

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Bibliographic Details
Edition:	First edition 2023.
Imprint:	New York, NY : Springer US : Imprint: Humana, 2023.
Description:	1 online resource (XI, 286 pages) : online resource.
Language:	English
Series:	Methods in Molecular Biology, 1940-6029 ; 2676 Methods in molecular biology (Clifton, N.J.) ; 2676.
Subject:	Amino acids. Genetic code. Amino acids Genetic code Laboratory manuals.
Format:	E-Resource Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/13588353

Hidden Bibliographic Details
Other authors / contributors:	Tsai, Yu-Hsuan., editor. Elsässer, Simon J., editor.
ISBN:	9781071632512 1071632515
Notes:	Includes index.
Summary:	This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.
Standard no.:	10.1007/978-1-0716-3251-2

Description
Item Description:	Includes index.
Physical Description:	1 online resource (XI, 286 pages) : online resource.
ISBN:	9781071632512 1071632515
ISSN:	1940-6029 ;