Genetically Incorporated Non-Canonical Amino Acids : Methods and Protocols /

Genetically Incorporated Non-Canonical Amino Acids : Methods and Protocols /

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Bibliographic Details
Edition:First edition 2023.
Imprint:New York, NY : Springer US : Imprint: Humana, 2023.
Description:1 online resource (XI, 286 pages) : online resource.
Language:English
Series:Methods in Molecular Biology, 1940-6029 ; 2676
Methods in molecular biology (Clifton, N.J.) ; 2676.
Subject:
Amino acids.
Genetic code.
Amino acids
Genetic code
Laboratory manuals.
Format: E-Resource Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/13588353
Hidden Bibliographic Details
Other authors / contributors:Tsai, Yu-Hsuan., editor.
Elsässer, Simon J., editor.
ISBN:9781071632512
1071632515
Notes:Includes index.
Summary:This detailed volume explores non-canonical amino acids (ncAAs) through their site-specific incorporation by genetic code expansion (GCE). The collection provides a broad resource of methods for implementing GCE in E. coli, mammalian cells, and animals, highlighting specific applications ranging from fluorescence labeling to photocontrol and the study of protein post-translational modification. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genetically Incorporated Non-Canonical Amino Acids: Methods and Protocols serves as an ideal source of methodologies that can be adapted and extended, migrated to different model systems, and combined in new ways to help explore a wide range of biological questions and to augment industrial and pharmaceutical protein engineering.
Standard no.:10.1007/978-1-0716-3251-2
Table of Contents:
  • Focused Engineering of Pyrrolysyl-tRNA Synthetase-Based Orthogonal Translation Systems for the Incorporation of Various Non-Canonical Amino Acids
  • Engineering Homogeneous Photoactive Antibodies Fragments
  • Repurposing Photosensitizer Proteins through Genetic Code Expansion to Facilitate Photo-Biocatalysis
  • Genetic Encoding of a Fluorescent Non-Canonical Amino Acid as a FRET Donor for Analysis of Deubiquitinase Activities
  • Creating Selenocysteine-Specific Reporters Using Inteins
  • Protein Expression with Biosynthesized Non-Canonical Amino Acids
  • Reprogramming Initiator and Nonsense Codons to Simultaneously Install Three Distinct Non-Canonical Amino Acids into Proteins in E. coli
  • Encoding Non-Canonical Amino Acids into Phage Displayed Proteins
  • Genetically Encoded Non-Canonical Amino Acids in Proteins to Investigate Lysine Benzoylation
  • Semi-Synthesis of Glutamine-Methylated Proteins Enabled by Genetic Code Expansion
  • Genetic Code Expansion in Mammalian Cells
  • Generation of Amber Suppression Cell Lines Using CRISPR-Cas9
  • Genetic Code Expansion in Mammalian Cells through Quadruplet Codon Decoding
  • Genetically Encoded 1,2-Aminothiol for Site-Specific Modification of a Cellular Membrane Protein via TAMM Condensation
  • Conformational GPCR BRET Sensors Based on Bioorthogonal Labeling of Non-Canonical Amino Acids
  • Selective Inhibition of Kinase Activity in Mammalian Cells by Bioorthogonal Ligand Tethering
  • Site-Specific Incorporation of Sulfotyrosine into Proteins in Mammalian Cells
  • Small Molecule Phosphine Activation of Protein Function in Zebrafish Embryos with an Expanded Genetic Code
  • Non-Canonical Amino Acid Incorporation in Mice.

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