Flow cytometry : first principles /

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Bibliographic Details
Author / Creator:Givan, Alice Longobardi
Imprint:New York : Wiley-Liss, c1992.
Description:xiv, 202 p. : ill. ; 23 cm.
Language:English
Subject:
Format: Print Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/1468740
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ISBN:0471560952
Notes:Includes bibliographical references (p. 171-174) and index.
Table of Contents:
  • 1. The Past as Prologue
  • 2. Setting the Scene
  • 3. Instrumentation: Beyond the Black Box. The Optical Bench. The Fluid System. Electronics. Sorting
  • 4. Information: Harnessing the Data. Data Storage. Data Analysis
  • 5. Seeing the Light: Lasers, Fluorochromes, and Filters. General Theory. The Illuminating Beam. Fluorochromes. Lenses, Filters, and Mirrors. Compensation
  • 6. Cells From Without: Lymphocytes and the Strategy of Gating. Lymphocytes. Staining for Surface Markers. Controls. Quantification. The Strategy of Gating
  • 7. Cells From Within: DNA and Molecular Biology. Fluorochromes for DNA Analysis. Ploidy. Cell Cycle Analysis. Two-Color Analysis for DNA and Another Parameter. Chromosomes. Molecular Biology. Cell Death
  • 8. Disease and Diagnosis: The Clinical Laboratory. The Hematology/Immunology Laboratory. Oncology/Pathology Laboratories. Transplantation. Comments
  • 9. Out of the Mainstream: Research Frontiers. Functional Assays. The Aquatic Environment. Animal Development. Microbiology and Gel Microdroplets
  • 10. Flowing On: The Flow Cytometry Laboratory of the Future.