HPLC of proteins, peptides, and polynucleotides : contemporary topics and applications /

The second volume in a continuing series "Analytical Techniques in Clinical Chemistry and Laboratory Medicine" that emphasizes techniques, procedures, and results rather than theory. The 22 chapters of this rather large book describe high-performance liquid chromatography (HPLC) methodology for a variety of biopolymers such as peptides, proteins, polynucleotides, membrane proteins, viruses, and monoclonal antibodies. In addition, there is a very useful discussion of high-performance capillary electrophoresis. Figures, diagrams, and tables are used extensively and there is an adequate number of up-to-date references at the end of each chapter. The writing style is uniformly clear, even though each chapter has a different author. The six-page index appears to be a little brief, considering the relatively large size of this monograph. The first chapter provides an excellent, nontechnical overview of present chromatographic theory as well as a look at the future of HPLC for the separation and purification of biopolymers. Because of this brief but clear introduction, one need not be an expert in chromatographic theory in order to understand the chapters that follow. Of value to anyone actively involved in the separation and purification of biopolymers and related species. Graduate level.-H. T. McKone, Saint Joseph College