Flow cytometry : a practical approach /
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| Edition: | 2nd ed. |
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| Imprint: | Oxford [England] ; New York : IRL Press at Oxford University Press, c1994. |
| Description: | xxiii, 282 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Practical approach series 142 |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/1705166 |
| Summary: | A flow cytometer measures physical and chemical characteristics of single cells or organelles and enables them to be identified, counted, and physically separated (sorted) for further study. Flow cytometry is little more than 20 years old and is still unfamiliar to many biologists. The rapid increase in the number of machines being installed has meant that there are many comparatively inexperienced workers trying to cope with the new technology. This book offers practical guidance in preparing and staining their samples. It should also enable more experienced practitioners who want to investigate a new application.The second edition covers similar ground to the first. For example, chapters on fluorescence technology, DNA analysis and the measurement of cell proliferation, chromosome analysis and sorting, membrane potential and pH, and the further applications to cell biology, have been updated and, where necessary, expanded. New authors have written enlarged chapters on immunofluorescence of surface markers and measurement of cytoplasmic and nuclear antigens. |
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| Physical Description: | xxiii, 282 p. : ill. ; 24 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: | 0199634629 0199634610 |
