Tissue in situ hybridization : methods in animal development /
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| Author / Creator: | Jowett, Trevor. |
|---|---|
| Imprint: | New York : Wiley ; Heidelberg : Spektrum, c1997. |
| Description: | viii, 128 p., 8 p. of plates : ill. (some col.) ; 26 cm. |
| Language: | English |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/3455264 |
Table of Contents:
- Principle of the Technique
- Probe Synthesis
- Fixation
- Hybridization to Whole-Mount Embryos
- Immunolocalization of Probes
- Alternative Enzyme Substrates
- Two-Color In Situ Hybridizations
- Two-Color In Situ Hybridization with Fluorochromes
- Embedding and Sectioning Whole-Mount Embryos
- In Situ Hybridization on Paraffin Wax Embedded Tissue Sections
- In Situ Hybridization on Cryostat Sections of Chick Embryos
- Whole-Mount In Situ Hybridization of Thick, Floating Tissue Sections
- Simultaneous Localization of Transcription and Translation Gene Products
- Special Considerations, Modifications, and Problem Troubleshooting
- Photomicrography
- Solutions, Reagents, Equipment, and Suppliers
- References
- Index
