Gene expression systems : using nature for the art of expression /

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Bibliographic Details
Imprint:	San Diego : Academic Press, c1999.
Description:	xii, 480 p. : ill. ; 24 cm.
Language:	English
Subject:	Gene expression. Prokaryotes -- Genetics. Insects -- Genetics. Transgenic animals -- Genetics. Eukaryotic cells. Gene Expression Regulation. Eukaryotic cells. Gene expression. Insects -- Genetics. Prokaryotes -- Genetics.
Format:	Print Book
URL for this record:	http://pi.lib.uchicago.edu/1001/cat/bib/3538738

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Other authors / contributors:	Fernandez, Joseph M. Hoeffler, James P.
ISBN:	0122538404 9780122538407
Notes:	Includes bibliographical references and index.
Summary:	"Recombinant gene expression is the fastest growing area in the study of molecular biology. By the time the Human Genome Project is completed (about 2002), several thousand sequences will be known, but the purpose of the resultant expression products will remain a mystery. Gene discovery requires efficient expression systems for determining the structure and function of gene products. Gene Expression Systems covers a variety of promoters and host organisms that researchers can tailor to their specific needs."--Jacket.

Table of Contents:

Contributors
Introduction: The Art of Expression
Purpose of This Book
Selecting a Suitable Expression System: Considerations
Genomics and the Future of Protein Expression Systems
Prokaryotic Expression Systems: Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse
Expression Vectors Employing the trc Promoter
Bacillus Expression: A Gram Positive Model
araB Expression System in Escherichia coli
Eukaryotic Expression Systems: Adenoviral Vectors for Protein Expression
Expression in the Methalotrophic Yeast Pichia pastoris
Recombinant Protein Expression in Pichia methanolica
Cytomegalovirus Promoter for Expression in Mammalian Cells
Inducible Mammalian Expression Systems
Protein Expression in Mammalian Cells Using Sindbis Virus
Expression in Insect Systems: Drosophila S2 System for Heterologous Gene Expression
Baculovirus Expression Vector System
Transgenic Expression: Recombinant Protein Expression in Transgenic Mice
Expression of Recombinant Proteins in the Milk of Transgenic Animals
Recombinant Protein Expression in Plants
Index

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