HIV protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, c1999.
Description:xiii, 421 p. : ill. ; 24 cm.
Language:English
Series:Methods in molecular medicine 17
Subject:
Format: Print Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/3731519
Hidden Bibliographic Details
Other authors / contributors:Michael, Nelson.
Kim, Jerome H.
ISBN:0896033694 (alk. paper)
Notes:Includes index

MARC

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300 |a xiii, 421 p. :  |b ill. ;  |c 24 cm. 
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440 0 |a Methods in molecular medicine  |v 17 
500 |a Includes index 
505 0 0 |g Pt. I.  |t Virology.  |g 1.  |t Isolation and Expansion of HIV from Cells and Body Fluids by Coculture /  |r James R. Lane.  |g 2.  |t Quantitative HIV Culture /  |r James R. Lane.  |g 3.  |t Quantitation of Cell-Free HIV by Reverse Transcriptase Activity /  |r James R. Lane.  |g 4.  |t Determination of Syncytium-Inducing Phenotype of Primary HIV-1 Isolates Using MT-2 Cells /  |r Mark K. Louder and John R. Mascola.  |g 5.  |t Determination of HIV-1 Chemokine Coreceptor Tropism Using Transduced Human Osteosarcoma (HOS) Cells /  |r Mark K. Louder and John R. Mascola.  |g 6.  |t Use of Luciferase Reporter Viruses for Studying HIV Entry /  |r Rong Liu, Benjamin Chen and Nathaniel R. Landau.  |g 7.  |t A Cell-Cell Fusion Assay to Monitor HIV-1 Env Interactions with Chemokine Receptors /  |r Almee L. Edinger and Robert W. Doms.  |g 8.  |t Methods of Culturing HIV-1 from Semen /  |r Bruce L. Gilliam --  |g Pt. II.  |t Molecular Biology.  |g 9.  |t Detection of HIV-1 Nucleic Acids by Southern Blotting /  |r Richard A. McDonald.  |g 10.  |t Detection of HIV-1 Nucleic Acids by Northern Blotting /  |r Richard A. McDonald and Christopher A. D. Smith.  |g 11.  |t Rapid Identification of Cloned HIV-1 Fragments /  |r Wei Dong, Anindita Kar Roy and Yen Li.  |g 12.  |t PCR Amplification and Cloning of Virtually Full-Length HIV-1 Provirus /  |r Mika Salminen.  |g 13.  |t Quantitation of HIV-1 gag DNA and RNA From Single Frozen Cell Pellets /  |r Maryanne Vahey, Sandra C. Barrick and Martin Nau.  |g 14.  |t Quantitative PCR for HIV-1 Proviral DNA /  |r Davide Zella and Paola Secchiero.  |g 15.  |t Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper /  |r Sharon A. Cassol, Stanley Read and Bruce G. Weniger [et al.].  |g 16.  |t Quantification of HIV-1 RNA in Dried Plasma Spots (DPS): A Field Approach to Therapeutic Monitoring /  |r Sharon A. Cassol, Francisco Diaz-Miltoma and Richard Pilon [et al.].  |g 17.  |t Quantitation of HIV-1 RNA in Genital Secretions /  |r Bruce L. Gilliam.  |g 18.  |t Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction /  |r Omar Bagasra, Lisa E. Bobroski and Mohammad Amjad [et al.].  |g 19.  |t Determination of the Promoter Activity of HIV-1 Using the Chloramphenicol Acetyltransferase Reporter Gene Assay /  |r Loretta Tse and Nelson L. Michael.  |g 20.  |t In Vitro Techniques for Studies of HIV-1 Promoter Activity /  |r Fabio Romerio and David M. Margolis.  |g 21.  |t Detection of Polymorphisms in the HIV-1 Coreceptor CCR5 Using Single-Strand Conformation Polymorphism /  |r Maureen P. Martin and Mary Carrington.  |g 22.  |t Sequence-Specific Priming as a Rapid Screen for Known Mutations /  |r Mary Carrington.  |g 23.  |t Quantitation of HIV-1 Entry Cofactor Expression /  |r James L. Riley and Richard G. Carroll.  |g 24.  |t Retrovirus-Mediated Gene Transduction of SupT1 Cells /  |r Jen-Tsun J. Lin and Jerome H. Kim.  |g 25.  |t Cloning of HIV Single-Strand DNA Binding Protein from Human Lymphocyte Lambda gt11 Expression Library with [[superscript 32]P]-Oligomers /  |r Sook-Jin Hur and Derhsing Lai.  |g 26.  |t Telomeric Terminal Restriction Fragment (TRF) /  |r Sumesh Kaushal --  |g Pt. III.  |t Humoral Immunology.  |g 27.  |t Serologic Analysis by Enzyme-Linked Immunosorbent Assay (ELISA) /  |r Melissa Krider and Lawrence D. Loomis-Price.  |g 28.  |t Quantitative Immunoblotting with Fusion Proteins /  |r Margaret M. Mitchell and Lawrence D. Loomis-Price.  |g 29.  |t Analysis of Antibody Interactions with HIV-1 Envelope Expressed on the Surface of Acutely Infected H9 Cells /  |r Thomas C. VanCott and Paul L. Hallberg.  |g 30.  |t Analysis of Antibody-Antigen Interactions Using Surface Plasmon Resonance /  |r Thomas C. VanCott.  |g 31.  |t Measurement of HIV-1 Specific and Total Antibody Secreting Cells by ELISPOT /  |r Frederik W. van Ginkel, John L. VanCott and Robert Kaminski [et al.].  |g 32.  |t Linear Epitope Mapping by the PEPSCAN Method /  |r Lawrence D. Loomis-Price.  |g 33.  |t Neutralization of HIV-1 Infection of Human Peripheral Blood Mononuclear Cells (PBMC): Antibody Dilution Method /  |r John R. Mascola.  |g 34.  |t Neutralization of HIV-1 Infection of Human Peripheral Blood Mononuclear Cells (PBMC): Infectivity Reduction Method /  |r John R. Mascola.  |g 35.  |t Collection and Processing of Mucosal Secretions from HIV-1 Infected Women /  |r Andrew W. Artenstein and Thomas C. VanCott.  |g 36.  |t Collection and Processing of Mucosal Secretions from Mice /  |r Robert W. Kaminski and Thomas C. VanCott --  |g Pt. IV.  |t Cellular Immunology.  |g 37.  |t Lymphocyte Proliferation Assay /  |r Karl V. Sitz and Deborah L. Birx.  |g 38.  |t HIV-1 Specific Cytotoxic T-Cell Assays /  |r Josephine H. Cox.  |g 39.  |t HIV-1 Specific Antibody-Dependent Cellular Cytotoxicity (ADCC) /  |r Josephine H. Cox.  |g 40.  |t Evaluation of Natural Killer Cell Activity /  |r Josephine H. Cox.  |g 41.  |t Generation and Expansion of HIV-1 CD4+ Antigen-Specific T Cell Lines /  |r Silvia Ratto-Kim.  |g 42.  |t Immunophenotyping and Assessment of Cell Function by Three-Color Flow Cytometry of Peripheral Blood Lymphocytes /  |r Stephen P. Perfetto and Gilbert McCrary.  |g 43.  |t Detection of Apoptosis in HIV-Infected Cell Populations using TUNEL /  |r Patrick J. Blair and Stephen P. Perfetto. 
650 0 |a AIDS (Disease)  |v Laboratory manuals. 
650 0 |a HIV (Viruses)  |v Laboratory manuals. 
650 0 |a HIV infections  |v Laboratory manuals. 
650 1 2 |a HIV Infections  |x prevention & control. 
650 1 2 |a Clinical Protocols. 
650 2 2 |a Molecular Biology  |x methods. 
650 2 2 |a Immunologic Techniques. 
650 2 2 |a Virology  |x methods. 
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650 7 |a HIV infections.  |2 fast  |0 http://id.worldcat.org/fast/fst00949871 
650 7 |a HIV (Viruses)  |2 fast  |0 http://id.worldcat.org/fast/fst00949837 
655 7 |a Laboratory manuals.  |2 fast  |0 http://id.worldcat.org/fast/fst01920776 
700 1 |a Michael, Nelson.  |0 http://id.loc.gov/authorities/names/n98801700  |1 http://viaf.org/viaf/39551076 
700 1 |a Kim, Jerome H.  |0 http://id.loc.gov/authorities/names/n86824515  |1 http://viaf.org/viaf/71567154 
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