HIV protocols /
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| Imprint: | Totowa, N.J. : Humana Press, c1999. |
|---|---|
| Description: | xiii, 421 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular medicine 17 |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/3731519 |
Table of Contents:
- Part I. Virology
- Isolation and Expansion of HIV from Cells and Body Fluids by Coculture Quantitative HIV Culture
- Quantitation of Cell-Free HIV by Reverse
- Transcriptase Activity
- Determination of Syncytium-Inducing
- Phenotype of Primary HIV-1 Isolates
- Using MT-2 Cells Determination of HIV-1 Chemokine
- Coreceptor Tropism
- Using Transduced Human Osteosarcoma (HOS) Cells
- Use of Luciferase
- Reporter
- Viruses for Studying
- HIV Entry A Cell-Cell Fusion Assay to Monitor
- HIV-1 Env Interactions with Chemokine
- Receptors
- Methods of Culturing
- HIV-1 from Semen
- Part II. Molecular Biology
- Detection of HIV-1 Nucleic Acids by Southern Blotting
- Detection of HIV-1 Nucleic Acids by Northern Blotting
- Rapid Identification of Cloned HIV-1 Fragments
- PCR Amplification and Cloning of Virtually
- Full-Length HIV-1 Provirus
- Quantitation of HIV-1gag DNA and RNA from Single Frozen Cell Pellets
- Quantitative PCR for HIV-1 Proviral DNA Diagnosis and Direct Automated Sequencing of HIV-1
- From Dried Blood Spots (DBS) Collected on Filter Paper
- Quantification of HIV-1 RNA in Dried Plasma Spots (DPS)
- A Field Approach to Therapeutic Monitoring
- Quantitation of HIV-1 RNA in Genital Secretions
- Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase
- Chain Reaction Omar Basgasra
- Determination of the Promoter
- Activity of HIV-1 Using the Chloramphenical Acetyltransferase
- Reporter Gene Assay In Vitro Techniques for Studies of HIV-1 Promoter Activity
- Detection of Polymorphisms in the HIV-1 Coreceptor CCR5
- Using Single-Strand Conformation Polymorphism Sequence-Specific P
- Priming as a Rapid Screen for Known Mutations
- Quantitation of HIV-1 Entry Cofactor
- Expression Retrovirus
- Mediated Grene Transduction of SupT1
- Cells Cloning of HIV
- Single-Strand DNA
- Binding Protein from Human Lymphocyte Lamda gt11
- Expression Library with P32-Oligomers Telomeric
- Terminal Restriction Fragment (TRF)
- Part III. Humoral Immunology
- Serologic Analysis by Enzyme-Linked Immunosorbent Assay (ELISA)
- Quantitative Immunoblotting with Fusion Proteins Analysis of Antibody Interactions with HIV-1 Envelope
- Expressed on the Surface of Acutely Infected H9
- Cells Analysis of Antibody-Antigen Interactions
- Using Surface Plasmon
- Resonance Measurement of HIV-1
- Specific and Total Antibody
- Secreting Cells by Elispot
- Linear Epitope Mapping by the Pepscan
- Method Neutralization of HIV-1
- Infection of Human Peripheral Blood Mononuclear Cells (PBMC)
- Antibody Dilution Method Neutralization of HIV-1
- Infection of Human Peripheral Blood Mononuclear Cells (PBMC)
- Infectivity Reduction Method Collection and Processing of Mucosal
- Secretions from HIV-1 Infected Women Collection and Processing of Mucosal Secretions from Mice
- Part IV. Cellular Immunology
- Lymphocyte Proliferation Assay HIV-1 Specific Cytotoxic T-Cell Assays
- HIV-1- Specific Antibody-Dependent Cellular Cytotoxicity (ADCC)
- Evaluation of Natural Killer Cell Activity
- Generation and Expansion of HIV-1 CD4+ Antigen-Specific T Cell Lines
- Immunophenotyping and Assessment of Cell Function by Three-Color Flow
- Cytometry of Peripheral Blood Lymphocytes
- Detection of Apoptosis in HIV
- Infected Cell Populations using Tunel
- Index
