Culture of animal cells : a manual of basic technique /
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| Author / Creator: | Freshney, R. Ian. |
|---|---|
| Edition: | 4th ed. |
| Imprint: | New York : Wiley-Liss, c2000. |
| Description: | xxvi, 577 p., [12] p. of plates : ill. (some col.) ; cm. |
| Language: | English |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/4253187 |
Table of Contents:
- Figures
- Color Plates
- Preface
- Abbreviations
- 1. Introduction
- Background
- Advantages of Tissue Culture
- Limitations
- Major Differences In Vitro
- Types of Tissue Culture
- 2. Biology of Cultured Cells
- The Culture Environment
- Cell Adhesion
- Cell Proliferation
- Differentiation
- Energy Metabolism
- Initiation of the Culture
- Evolution of Cell Lines
- The Development of Continuous Cell Lines
- Origin of Cultured Cells
- 3. Design and Layout
- Planning
- Construction and Services
- Layout
- 4. Equipment
- Requirements of a Tissue Culture Laboratory Essential Equipment
- Beneficial Equipment
- Useful Additional Equipment
- Consumable Items
- 5. Aseptic Technique
- Objectives of Aseptic Technique
- Elements of Aseptic Environment
- Sterile Handling
- Laminar Flow
- Standard Procedure
- Apparatus and Equipment
- 6. Safety
- Risk Assessment
- General Safety
- Fire
- Radiation
- Biohazards
- 7. Culture Vessels
- The Substrate
- Choice of Culture Vessel
- Specialized Systems
- Treated Surfaces
- 8. Media
- Development of Media
- Physicochemical Properties
- Balanced Salt Solutions
- Complete Media
- Serum
- Selection of Medium and Serum
- Other Supplements
- 9. Serum-Free Media
- Disadvantages of Serum
- Advantages of Serum-Free Media
- Disadvantages of Serum-Free Media
- Replacement of Serum
- Selection of Serum-Free Medium
- Development of Serum-Free Medium
- Preparation of Serum-Free Medium
- Conclusions
- 10. Preparation and Sterilization
- Apparatus
- Reagents and Media
- Control, Testing, and Storage of Media
- 11. Primary Culture
- Types of Primary Cell Culture
- Isolation of the Tissue
- Primary Culture
- 12. Cell Lines
- Nomenclature
- Subculture and Propagation
- Immortalization of Cell Lines
- Cell Line Designations
- Selection of Cell Line
- Routine Maintenance
- 13. Cloning and Selection
- Cloning
- Stimulation of Plating Efficiency
- Suspension Cloning
- Isolation of Clones
- Replica Plating
- Selective Inhibitors
- Isolation of Genetic Variants
- Interaction with Susbstrate
- 14. Cell Separation
- Cell Density and Isopyknic Sedimentation
- Antibody-Based Techniques
- Magnetic Sorting
- Cell Size and Sedimentation Velocity
- Centrifugal Elutriation
- Fluorescence-Activated Cell Sorting
- Other Techniques
- Beginner's Approach to Cell Separation
- 15. Characterization
- The Need for Characterization
- Morphology
- Chromosome Analysis
- DNA Content
- RNA and Protein
- Enzyme Activity
- Antigenic Markers
- Differentiation
- Authentication
- 16. Differentiation
- Expression of In Vivo Phenotype
- Stages of Commitment and Differentiation
- Proliferation and Differentiation
- Commitment and Lineage
- Markers of Differentiation
- Induction of Differentiation
- Differentiation and Malignancy
- Practical Aspects
- 17. Transformation
- Role in Cell Line Characterization
- What is Transformation?
- Genetic Instability
- Immortalization
- Aberrant Growth Control
- Tumorigenicity
- 18. Contamination
- Sources of Contamination
- Types of Microbial Contamination
- Monitoring for Contamination
- Eradication of Contamination
- Cross-Contamination
- Conclusions
- 19. Cryopreservation
- Need for Cryopreservation
- Preservation
- Cell Banks
- Transporting Cells
- 20. Quantitation
- Cell Counting
- Cell Weight
- DNA Content
- Protein
- Rates of Synthesis
- Preparation of Samples for Enzyme Assay and Immunoassay
- Cytometry
- Replicate Sampling
- Cell Proliferation
- Plating Efficiency
- Labeling Index
- Cell Cycle Time (Generation Time)
- Cell Migration
- 21. Cytotoxicity
- Introduction
- In Vitro Limitations
- Nature of the Assay
- Anticancer Drug Screening
- Transformation
- Inflammation
- 22. Specialized Cells
- Epithelial Cells
- Mensenchymal Cells
- Neuroectodermal Cells
- Hematopoietic Cells
- Gonads
- 23. Tumor Cells
- Sampling
- Disaggregation
- Primary Culture
- Characterization
- Development of Cell Lines
- Selective Culture
- Specific Tumor Types
- 24. Organotypic Culture
- Cell Interaction and Phenotypic Expression
- Organ Culture
- Histotypic Culture
- Filter-Well Inserts
- Cultures of Neuronal Aggregates
- Organotypic Culture
- 25. Scale-Up
- Scale-up in Suspension
- Scale-up in Monolayer
- 26. Specialized Techniques
- Lymphocyte Preparation
- Autoradiography
- Time-Lapse Recording
- Confocal Microscopy
- Cell Synchrony
- Culture of Amniocytes
- Culture of Cells from Poikilotherms
- 27. Molecular Techniques
- Molecular Biology in Cell Culture
- In Situ Molecular Hybridization
- Somatic Cell Fusion
- Production of Monoclonal Antibodies
- DNA Transfer
- 28. Problem Solving
- Slow Cell Growth
- Choice of Medium
- Unstable Reagents
- Purity of Constituents
- Plastics
- Glassware
- Microbial Contamination
- Chemical Contamination
- Primary Culture
- Cloning
- Differentiation
- Feeding
- Subculture
- Cross-Contamination
- Cryopreservation
- Granularity of Cells
- Cell Counting
- Viability
- In Conclusion
- Reagent Appendix
- Trade Index
- Glossary
- References
- Index
