Techniques in molecular medicine /
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| Imprint: | Berlin ; New York : Springer, c1999. |
|---|---|
| Description: | xii, 387 p. : ill. (some col.) ; 25 cm. |
| Language: | English |
| Series: | Springer lab manual |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/4274050 |
Table of Contents:
- Pt. I. Molecular Biology Techniques. Ch. 1. Structure and Function of the Human Genome / Friedhelm Hildebrandt. Ch. 2. Enzymatic Modification of DNA and RNA / Kai-Olaf Netzer. Ch. 3. Purification and Nucleic Acids From Eukaryotic Cells / Hans Gerd Nothwang and Friedhelm Hildebrandt. Ch. 4. Electrophoresis of DNA and RNA Fragments / Feng Qian and Gregory G. Germino. Ch. 5. Single-Strand Conformation Polymorphism (SSCP) Analysis / Feng Qian and Gregory G. Germino. Ch. 6. Denaturing Gradient Gel Electrophoresis / Kai-Olaf Netzer. Ch. 7. Introduction of Pulsed-Field Gel Electrophoresis (PFGE) / Feng Qian and Gregory G. Germino. Ch. 8. Pulsed-Field Gel Electrophoresis: Protocols / Gudrun A. Rappold, Karin Ried and Albrecht Klink / [et al.]. Ch. 9. Radioactive Labeling of DNA and RNA Probes / Peter Igarashi. Ch. 10. Hybridization Methods (Southern and Northern Blotting) / Kai-Olaf Netzer. Ch. 11. Specimen Preparation Techniques for Cytogenetic Analysis by Fluorescence in situ Hybridization / Stefan Joos, Martin Bentz and Anton H. N. Hopman / [et al.]. Ch. 12. Chromosome Analysis by Fluorescence in situ Hybridization / Martin Bentz, Stefan Joos and Peter Lichter. Ch. 13. Sequencing / Klaus Deichmann. Ch. 14. Polymerase Chain Reaction / Friedhelm Hildebrandt and Iva Singh-Sawhney. Ch. 15. Cloning Vectors / Annette Deichmann and Klaus Deichmann
- Pt. II. Strategies for Gene Isolation and Characterization. Ch. 16. Cloning Strategies: An Overview / Friedhelm Hildebrandt. Ch. 17. Genomic Libraries / Luitz F. Onuchic and Gregory G. Germino. Ch. 18. cDNA Libraries / Luitz F. Onuchic and Gregory G. Germino. Ch. 19. cDNA Library Screening / Johann Peter Hossle. Ch. 20. Expression Cloning with Xenopus Oocytes / Daniel Markovich, Andreas Werner and Heini Murer. Ch. 21. Transgenic Animals / Eckart Schott, Martin Paul and Detlev Ganten. Ch. 22. Expression in Eukaryotic Cells: Gene Transfer Using Retroviral Vectors / Martin Marx. Ch. 23. Positional Cloning and Linkage Analysis / Friedhelm Hildebrandt and Heymut Omran
- Pt. III. Protein Methods. Ch. 24. Protein Techniques / Robert F. Reilly.
