Molecular pathology protocols /

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Bibliographic Details
Imprint:Totowa, N.J. : Humana Press, 2001.
Description:xiv, 491 p. ; 24 cm.
Language:English
Series:Methods in molecular medicine 49
Subject:
Format: Print Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/4357412
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Other authors / contributors:Killeen, Anthony A.
ISBN:0896036812 (alk. paper)
Notes:Includes bibliographical references and index.
Table of Contents:
  • Preface
  • Contributors
  • 1. DNA Extraction from Paraffin-Embedded Tissues
  • 2. DNA Extraction from Fresh or Frozen Tissues
  • 3. RNA Extraction from Fresh or Frozen Tissues
  • 4. Single-Strand Conformation Polymorphism Analysis of Mutations in Exons 4-8 of the TP53 Gene
  • 5. Cleavase Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection
  • 6. Detection of Telomerase by In Situ Hybridization and by the Polymerase Chain Reaction-Based Telomerase Activity Assay
  • 7. Detection of Microsatellite Instability
  • 8. Polymerase Chain Reaction Clonality Assays Based on X-Linked Genes
  • 9. Fluorescent In Situ Hybridization: Evaluation for Ploidy and Gene Amplification
  • 10. HER-2/neu Oncogene Amplification Determined by Fluorescence In Situ Hybridization
  • 11. A Nested Reverse Transcription-Polymerase Chain Reaction Assay to Detect BCR/abl
  • 12. Detection of t(15;17)(q24;q21), inv(16)/t(16;16)(p13;q22), and t(8;21)(q22;q22) Anomalies in Acute Myeloid Leukemia
  • 13. Detection of t(14;18)(q32;q21)-Associated BCL-2/J[subscript H] Gene Fusion in Non-Hodgkin Lymphoma
  • 14. Detection of Breast Cancer Cells Using Immunomagnetic Beads and Reverse Transcriptase Polymerase Chain Reaction
  • 15. Molecular Detection of Circulating Prostate Cancer Cells
  • 16. Methods to Detect Clonal Gene Rearrangements in Lymphomas and Leukemias
  • 17. Monitoring of Bone Marrow Transplant Engraftment
  • 18. Direct Molecular Diagnosis of Multiple Endocrine Neoplasia Type 1
  • 19. Molecular Detection of Multiple Endocrine Neoplasia Type 2
  • 20. Assay for Detecting the l1307K Susceptibility Allele within the Adenomatous Polyposis Coli Gene
  • 21. Detection of Human Papillomaviruses by Polymerase Chain Reaction and In Situ Hybridization
  • 22. Molecular Methods for Detecting Epstein-Barr Virus (Part I): In Situ Hybridization to Epstein-Barr Virus-Encoded RNA (EBER) Transcripts
  • 23. Molecular Methods for Detecting Epstein-Barr Virus (Part II): Structural Analysis of Epstein-Barr Virus DNA as a Marker of Clonality
  • 24. Molecular Methods for Detecting Epstein-Barr Virus (Part III): EBV Viral Load by Competitive Polymerase Chain Reaction
  • 25. Molecular Detection of Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus-8
  • 26. Diagnostic Applications of Quantitative Polymerase Chain Reaction for Cytomegalovirus
  • 27. A Colorimetric Microtiter Plate Polymerase Chain Reaction System That Detects Herpes Simplex Virus in Cerebrospinal Fluid and Discriminates Genotypes 1 and 2
  • 28. Detection and Typing of Hepatitis C Virus
  • 29. Detection and Speciation of Mycobacteria in Formalin-Fixed, Paraffin-Embedded Tissue Sections
  • 30. Ultrasensitive Quantiation of Human Immunodeficiency Virus Type 1 RNA in Plasma by the AMPLICOR and COBAS AMPLICOR HIV-1 MONITOR Tests
  • 31. Molecular Diagnosis of Hereditary Thrombotic Disorders
  • 32. Prenatal Genotyping of the RhD Locus to Identify Fetuses at Risk for Hemolytic Disease of the Newborn
  • 33. Molecular Diagnosis of Hereditary Hemochromatosis
  • 34. Genotyping of Apolipoprotein E
  • 35. Genotyping for Functionally Important Human CYP2D6*4 (B) Mutation Using TaqMan Probes
  • Index