Flow cytometry and cell sorting /
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| Edition: | 2nd ed. |
|---|---|
| Imprint: | Berlin ; New York : Springer, c2000. |
| Description: | xxiii, 355 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Springer lab manual |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/4367766 |
Table of Contents:
- Preface
- Part I. Flow Cytometry: Operation of a Flow Cytometer
- Part II. Immunofluorescence
- 2. Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies
- 3. Immunofluorescence: Basic Considerations
- 4. Multicolor Immunofluorescence Analysis
- 5. Combined Intracellular and Surface Staining: Immunofluorescence of Cytokines in
- 6. Cytometry and Sorting of Live Cells According to Their Secreted Products
- 7. Cytometry of Rare Surface Molecules by Magnetofluorescent Liposomes
- 8. Scatchard Analysis by Flow Cytometry
- 9. Microbead Assay for Quantification of Neuronal Adhesion Molecule Interaction
- Part III. DNA and Proliferation
- 10. Preparation and Staining of Fixed Cells for High-Resolution DNA Analysis
- 11. Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression
- 12. High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique
- Part IV. Cellular Activation and Biochemistry
- 13. Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium- Manfred Kubbies
- 14. Detection and Purification of Rare Responders
- 15. Biochemical Parameters of Cell Function
- 16. The FACS-Gal Assay
- 17. Ligand Acidification
- Part V. Cell Sorting
- 18. Powerful Preselection
- 19. Magnetic Selection of Transfected Cells
- 20. High Gradient Magnetic Cell Sorting
- 21. Setup of a Flow Sorter
- 22. Sorting of Rare Cells
- 23. Fluorescence-Activated Chromosome Sorting
- 24. Analysis and Sorting of Plant Chromosomes
- 25. Large Particle Sorting
- Part VI. Saftey
- 26. Biological and Laser Safety
- Appendices: Glossary
- Suppliers
- Cytometry on the Internet
