Functional analysis of bacterial genes : a practical manual /
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| Imprint: | Chichester [West Sussex, U.K.] ; New York : John Wiley, c2001. |
|---|---|
| Description: | xxiii, 373 p. : ill. ; 25 cm. |
| Language: | English |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/4416729 |
Table of Contents:
- List of Contributors
- Foreword
- Preface
- Safety
- 1. Aims of the Functional Analysis Project
- 2. In silico Genome Analysis
- 3. Inactivation of Bacillus subtilis Genes Without Known Functions
- 4. Analysis of Essential Genes
- 5. Transcriptional Analysis of Large Regions of the Bacterial Genome
- 5A. Useful Methods for Transcriptional Analysis
- 5B. Northern Blot Analysis with non-RI Detection Systems
- 6. Micado, an Integrative Database Dedicated to the Functional Analysis of Bacillus subtilis and Microbial Genomics
- 6A. Phenotype Responses and Reporter Gene Activity from the Systematic Functional Analysis of Bacillus subtilis Unknown Genes
- 7. The Contribution of the EC Consortium to the Two-dimensional Protein Index of Bacillus subtilis
- 8. Whole-Cell Fingerprinting-Pyrolysis Mass Spectrometry
- 9. Metabolism of Small Molecules and Inorganics
- 9A. Screening for Genes Involved in Nucleotide and Nitrogen Metabolism
- 9B. Screening for Growth and lacZ Reporter Gene Induction with Metal Ions and Chelators, Amino Acid Analogs, Antibiotics, Inhibitors of Oxidative Phosphorylation and Miscellaneous Stress Factors
- 10. Nucleotide Metabolism
- 11. Utilization of Small Carbon-containing Molecules by Bacillus subtilis
- 11A. Screening for Genes Involved in the Metabolism of Small Carbon-containing Molecules
- 12. Nitrogen Sources
- 12A. Induction or Repression by Nitrogen Sources
- 13. Glucose Regulation in Bacillus subtilis
- 13A. Catabolite Repression by Glucose
- 14. Cell Processes and Macromolecule Metabolism: a Quest for Genes Involved in Genetic Competence, and DNA Recombination and Repair
- 14A. Cell Processes and Macromolecule Metabolism: Methods for the Selection of Mutants in Genetic Competence, DNA Recombination and Repair and Protein Secretion
- 15. Molecular Tools for the Identification of Mutations Affecting Protein Secretion
- 15A. Screening for Mutants Defective in Secretion
- 16. Genes Affecting Motility
- 16A. Screening for Motility
- 17. Differentiation of Bacillus subtilis
- 17A. Methods for Rapid Screening of Properties: Spore Resistance and Germination
- 18. Genes Affecting the Cell Envelope
- 18A. Analysis of Cell Envelope Components
- 19. Peptidoglycan Structure and Structural Determination in Vegetative Cells of Bacillus subtilis
- 20. Cell Cycle Gene Analysis
- 20A. Identification and Analysis of Cell Cycle Genes
- 21. Endospore Formation
- 21A. Isolation and Characterization of Genes Affecting Sporulation or the Cell Cycle
- 22. Selection and Characterization of Genes Affecting Germination
- 23. The Oxidative Stress Response
- 23A. Screening for Increased Sensitivity or Resistance and Level of lacZ Reporter Gene Expression in Response to the Oxidizing Agents Hydrogen Peroxide and Methyl Viologen
- 24. The Osmotic Shock Response
- 24A. Salt Stress
- 25. Phosphate Regulation
- 25a. Screening for Mutants Affected in their Response to Phosphate
- 26. The Heat Shock Response
- 27. The Cold Shock Response
- 28. pH-Inducible Genes
- 28A. Analysis of the Mutant Collection for Defects in their Temperature and pH Shock Response
- 28B. Screening for Growth and lacZ Reporter Gene Induction or Repression under Glucose and Phosphate Starvation and under Heat, Oxidative and Osmotic Stress
- 29. List of Bacillus subtilis Unknown Genes with a Phenotype Determined by the Functional Analysis Project
- 30. List of Bacillus subtilis Genes of Known Function
- Appendix 1. List of Bacillus subtilis Mutants Constructed by the European Functional Analysis Project
- Appendix 2. List of Bacillus subtilis Mutants Constructed by the Japanese Functional Analysis Project
- Appendix 3. List of Selected Suppliers
- Appendix 4. Glossary
- Index
