Epitope mapping : a practical approach /
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| Imprint: | Oxford ; New York : Oxford University Press, 2001. |
|---|---|
| Description: | xviii, 284 p. : ill. ; 25 cm. |
| Language: | English |
| Series: | The practical approach series Practical approach series. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/4653351 |
MARC
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| 082 | 0 | 0 | |a 616.07/92 |2 21 |
| 245 | 0 | 0 | |a Epitope mapping : |b a practical approach / |c edited by Olwyn M.R. Westwood, Frank C. Hay. |
| 260 | |a Oxford ; |a New York : |b Oxford University Press, |c 2001. | ||
| 300 | |a xviii, 284 p. : |b ill. ; |c 25 cm. | ||
| 336 | |a text |b txt |2 rdacontent |0 http://id.loc.gov/vocabulary/contentTypes/txt | ||
| 337 | |a unmediated |b n |2 rdamedia |0 http://id.loc.gov/vocabulary/mediaTypes/n | ||
| 338 | |a volume |b nc |2 rdacarrier |0 http://id.loc.gov/vocabulary/carriers/nc | ||
| 490 | 1 | |a The practical approach series | |
| 504 | |a Includes bibliographical references and index. | ||
| 505 | 0 | 0 | |g 1. |t An introduction to epitope mapping / |r Olwyn M. R. Westwood and Frank C. Hay -- |t Environmental conditions can influence protein structure -- |t Lessons from an historical perspective -- |t Synthetic peptide technologies for epitope mapping -- |t Chemical modification of antigens -- |t Site-directed mutagenesis as a tool for epitope mapping -- |t Hybridoma technology and epitope analysis -- |t Generating monoclonal antibody -- |t Phage display libraries -- |t Carbohydrates and their significance when epitope mapping -- |t Approaches to epitope mapping -- |g 2. |t Multiple Pin Peptide Scanning ("Pepscan") / |r Nazira Sumar -- |t Solid phase peptide synthesis -- |t Multiple peptide synthesis on pins -- |t Testing of antibody epitopes -- |t Results and data analysis -- |t Further analysis -- |t Applications of Pepscan -- |t Other systems for epitope analysis -- |g 3. |t Methodological tips for human T cell epitope mapping when using pin technology peptide arrays / |r David A. Mutch and Olwyn M. R. Westwood -- |t Historic foundations to large scale T cell epitope mapping -- |t Fundamental considerations -- |t Peptide synthesis considerations -- |t Use of pin peptide in human PBMC proliferation and cell culture assays -- |t Data analysis using the ALLOC algorithm -- |t Example of assay conditions for large scale mapping of T cell epitopes. |t Published methods to detect cellular antigenic stimulation -- |g 4. |t Combined B cell and T cell epitopes / |r Sowsan F. Atabani -- |t Molecular mapping of antigenic and immunogenic epitopes -- |t In vivo analysis of immunogenicity and antigenicity -- |g 5. |t CTL epitopes / |r Tim Elliott and John S. Haurum -- |t Indirect methods of CTL epitope identification using synthetic peptides -- |t Direct identification of CTL epitopes. |g App. 1. |t Class I MHC peptide motifs -- |g App. 2. |t Class I MHC alleles expressed by TAP-deficient cell lines -- |g App. 3. |t Monoclonal antibodies specific for class I MHC molecules -- |g App. 4. |t Index peptides suitable for modification -- |g 6. |t The design, synthesis, and characterization of molecular mimetics / |r Ian T. W. Matthews -- |t Design synthesis, and characterization of peptoid oligomers -- |t Further oligomeric peptide mimetics -- |t Non-oligomeric peptide mimetics -- |g 7. |t Generating monoclonal antibody probes and techniques for characterizing and localizing reactivity to antigenic determinants / |r Paul N. Nelson -- |t Immunization strategies -- |t Hybridization and culture of hybridomas -- |t Monoclonal purification -- |t Monoclonal conjugation -- |t Techniques for screening and characterizing mAb reactivity to antigenic determinants -- |t Physicochemical and chemical modification, pepsin digestion -- |t Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting -- |t Protein determination -- |g 8. |t Epitope mapping of carbohydrate binding proteins using synthetic carbohydrates / |r U. J. Nilsson and G. Magnusson -- |t Synthetic carbohydrate analogues -- |t Measuring carbohydrate-protein interactions -- |t Data interpretation -- |g 9. |t Phage display libraries / |r Samantha Williams, Paul van der Logt and Volker Cermaschewski -- |t Case study: identification of dominant epitopes -- |g 10. |t Site-directed mutagenesis in epitope mapping / |r Samuel Perdue -- |t General approaches to mutagenesis in epitope mapping: techniques in site-directed mutagenesis. |
| 650 | 0 | |a Antigenic determinants |v Laboratory manuals. | |
| 650 | 7 | |a Antigenic determinants. |2 fast |0 http://id.worldcat.org/fast/fst00810521 | |
| 655 | 7 | |a Laboratory manuals. |2 fast |0 http://id.worldcat.org/fast/fst01920776 | |
| 700 | 1 | |a Westwood, Olwyn M. R. |0 http://id.loc.gov/authorities/names/n89230813 |1 http://viaf.org/viaf/8979578 | |
| 700 | 1 | |a Hay, Frank C. |0 http://id.loc.gov/authorities/names/n88008426 |1 http://viaf.org/viaf/94475740 | |
| 830 | 0 | |a Practical approach series. |0 http://id.loc.gov/authorities/names/n84709852 | |
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| 928 | |t Library of Congress classification |a QR186.3 .E65 2001 |l JCL |c JCL-Sci |i 4604859 | ||
| 927 | |t Library of Congress classification |a QR186.3 .E65 2001 |l JCL |c JCL-Sci |e CL27 |e CRERAR |b 59486172 |i 7261461 | ||
