Telomeres and telomerase : methods and protocols /
Saved in:
| Imprint: | Totowa, N.J. : Humana Press, c2002. |
|---|---|
| Description: | xi, 221 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 191 Methods in molecular biology (Clifton, N.J.) ; v. 191. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/4698859 |
Table of Contents:
- Preface
- Contributors
- 1. Introduction to Telomeres and Telomerase
- 2. Detection of Chromosome Ends by Telomere FISH
- 3. Telomere Length Distribution: Digital Image Processing and Statistical Analysis
- 4. Analysis of Telomerase RNA Gene Expression by In Situ Hybridization
- 5. Relative Gene Expression in Normal and Tumor Tissue by Quantitative RT-PCR
- 6. Quantitative Detection of Telomerase Components by Real-Time, Online RT-PCR Analysis with the LightCycler
- 7. Standard TRAP Assay
- 8. Stretch PCR Assay
- 9. Fluorescent Detection of Telomerase Activity
- 10. Nonradioactive Detection of Telomerase Activity Using a PCR-ELISA-Based Telomeric Repeat Amplification Protocol
- 11. In Situ TRAP Assay Detection of Telomerase Activity in Cytological Preparations
- 12. Biotinylated Primer for Detecting Telomerase Activity Without Amplification
- 13. Whole-Cell and Microcell Fusion for the Identification of Natural Regulators of Telomerase
- 14. Screening with COMPARE Analysis for Telomerase Inhibitors
- 15. Telomerase as a Therapeutic Target: Therapeutic Potential of Telomerase Inhibitors
- Index
