GTPase protocols : the Ras superfamily /
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| Imprint: | Totowa, N.J. : Humana Press, c2002. |
|---|---|
| Description: | xi, 267 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 189 Methods in molecular biology (Clifton, N.J.) ; v. 189. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/4698860 |
Table of Contents:
- Preface
- Contributors
- Part I.. General Protocols
- 1. The GTPase Cycle: How Dominant Inhibitory Mutants Block the Biological Functions of Small GTPases
- 2. Preparation of GTPases for Structural and Biophysical Analysis
- 3. Using cDNA-Representational Difference Analysis (cDNA-RDA) in Combination with Microarrays to Identify Rac Regulated Genes
- 4. Fluorescence Methods in the Study of Small GTP-Binding Proteins
- Part II.. Specific Methods
- Ras Family GTPases
- 5. Ras and Rac as Activators of Reactive Oxygen Species (ROS)
- 6. Interfering with Ras Signaling Using Membrane-Permeable Peptides or Drugs
- 7. A Ras-Based Module to Generate [superscript 32]P-Labeled Fusion Proteins for Blot Overlays
- Rho Family GTPases
- 8. Determination of the Activity of Rho-Like GTPases in Cells
- 9. Introduction of Dominant Inhibitory Proteins Directed Against ROK and MRCK Kinases
- 10. Effects of Rho Family GTPases on Cell-Cell Adhesion
- 11. Cell Motility and Invasion Assays
- Rab Family GTPases
- 12. Isolation of Regulatory Proteins for the Rab3 Subfamily GTPases
- 13. Analysis and Preparation of Stable Complexes between Rab GTPases, Rab Escort Protein, and Rab Geranylgeranyl Transferase
- Arf Family GTPases
- 14. Preparation of Myristoylated Arf1 and Arf6 Proteins
- 15. ARF-Directed Guanine-Nucleotide-Exchange (GEP) Proteins
- 16. Arf6 and its Role in Cytoskeletal Modulation
- 17. ARF GTPase-Activating Protein 1
- Ran GTPases
- 18. The Use of Permeabilized Cell Systems to Study Nuclear Transport
- 19. Analysis of Nuclear Protein Import and Export In Vitro Using Fluorescent Cargoes
- 20. Characterization of the Effects of RanGTP on the Microtubule Cytoskeleton
- Index
