Oxidants and antioxidants : ultrastructure and molecular biology protocols /
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| Imprint: | Totowa, N.J. : Humana Press, c2002. |
|---|---|
| Description: | xvi, 356 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 196 Methods in molecular biology (Clifton, N.J.) ; v. 196. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/4714264 |
Table of Contents:
- Preface
- Contributors
- Part I.. Techniques for Histological Identification
- 1. Cytochemical Localization of H[subscript 2]O[subscript 2] in Biological Tissues
- 2. Localization of Intracellular Lipid Hydroperoxides Using Tetramethylbenzidine Reaction for Transmission Electron Microscopy
- 3. The Immunohistochemical Localization of Glutathione Peroxidase
- 4. 4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody
- 5. Immunohistochemical Detection of Protein Oxidation
- 6. Indirect Immunofluorescence Detection of Protein-Bound 4-Hydroxynonenal in Tissue Sections and Isolated Cells
- Part II.. Techniques for Subcellular Localization
- 7. Methods for Studying the Binding of Advanced Glycated Proteins to Receptors for Advanced Glycation Endproducts (AGE Receptors)
- 8. Measurement of Blue Fluorescence as a Protein Marker for Oxidized Membranes
- 9. X-Ray Diffraction Analysis of Membrane Structure Changes with Oxidative Stress
- 10. Detection of Estrogen Receptor by In Situ Hybridization
- 11. Tumor Necrosis Factor-[alpha] Quantification and Expression by In Situ Hybridization
- Part III.. Techniques for Molecular Biology
- 12. Translocation of p47[superscript phox] and Activation of NADPH Oxidase in Mononuclear Cells
- 13. Activation of Nuclear Factor-[kappa]B (NF-[kappa]B) in Mononuclear Cells (MNC)
- 14. Assay of Intracellular Hydrogen Peroxide Generation in Activated Individual Neutrophils by Flow Cytometry
- 15. DNA-Binding Activity of Hypoxia-Inducible Factors (HIFs)
- 16. Analysis of Heat-Shock Transcription Factor and Element-Binding Activity
- 17. Slot-Blot Hybridization in Studying Gene Expression During Oxygen Deprivation
- 18. An Expression System for a Transporter of Iron and Other Metals
- 19. Analysis of Gene Expression Following Oxidative Stress
- 20. Measurement of Immunoglobulin G Oxidation by Western-Blot Analysis
- 21. Thioredoxin and Redox Regulation of the Nuclear Receptor
- 22. Analysis of Phospholipid Hydroperoxide Glutathione Peroxidase mRNA
- 23. Expression of Human Phospholipid Hydroperoxide Glutathione Peroxidase
- Part IV.. Techniques for In Vivo/Ex Vivo/In Vitro Applications
- 24. Superoxide Production in the Islet of Langerhans Detected by the MCLA Chemiluminescence Method
- 25. In Vivo Detection of Transition Metals and Nitrosyl-Heme Complexes Using Ex Vivo Electron Paramagnetic Resonance Spectroscopy
- 26. Spectral-Spatial Electron Paramagnetic Resonance Imaging (EPRI) in Skin Bipsies at 9.5 GHz
- 27. In Vivo In Situ Detection of Nitric Oxide Using Low-Frequency EPR Spectroscopy
- 28. Introduction of NOS II Gene into Primary Cultures of Bovine and Human Endothelial Cells
- 29. Detection of Peroxynitrite-Induced Protein and DNA Modifications
- 30. Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-Nonenal and Lysine
- 31. Liver Slice Technology as an in Vitro Model for Metabolic and Toxicity Studies
- 32. Cytofluorescence Techniques for the Visualization of Distinct Pools of Protein Thiols at the Single Cell Level
- 33. Preparation of Microspheres and Incorporation of Lipid Hydroperoxide for Sustained Release Studies
- 34. Measurement of Carotenoids in the Living Primate Eye Using Resonance Raman Spectroscopy
- 35. Interstitial Photodynamic Therapy with Moving Exposure Fiber
- 36. Statistical Analysis: Receiver Operating Characteristic (ROC) Curve for Lipid Peroxidation
- Index
