Assessment of mammalian embryo quality : invasive and non-invasive techniques /

Assessment of mammalian embryo quality : invasive and non-invasive techniques /

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Bibliographic Details
Author / Creator:Soom, Ann van.
Imprint:Dordrecht ; Boston : Kluwer Academic Publishers, c2002.
Description:xxvii, 406 p. : ill. (some col.) ; 25 cm.
Language:English
Subject:
Mammals -- Embryology.
Mammals -- Embryology.
Format: Print Book
URL for this record:http://pi.lib.uchicago.edu/1001/cat/bib/4808071
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Other authors / contributors:Boerjan, Marleen.
ISBN:1402005814 (alk. paper)
Notes:Includes bibliographical references and index.
Table of Contents:
  • List of contributors
  • Preface
  • Introduction: Only the best conceptus will do! But what does best mean?
  • Chapter 1. Human embryo morphology and developmental capacity
  • 1.. Introduction
  • 2.. EggCyte Embryology Database
  • 3.. Zygote Morphology
  • 4.. Cleavage Stage embryos
  • 5.. Morulae and Blastocysts
  • 6.. Conclusions and future directions
  • Chapter 2. Morphological evaluation of embryos in domestic species
  • 1.. Introduction
  • 2.. Developmental processes in embryos
  • 3.. Classification of embryos by stage of development
  • 4.. Classification of embryos by quality grade
  • 5.. Relationship between embryo morphology evaluation and pregnancy rates
  • 6.. Factors affecting embryo evaluation
  • 7.. Embryo morphology in other domestic species
  • 8.. Conclusion
  • Chapter 3. Metabolic markers of embryo viability
  • 1.. Introduction
  • 2.. General characteristics of embryo metabolism
  • 3.. Non-invasive techniques of evaluation
  • 4.. Evaluation of embryo viability after embryo manipulation
  • 5.. Conclusions
  • Chapter 4. Embryo quality and freezing tolerance: cryopreservation of human embryos
  • 1.. Introduction
  • 2.. Selection of embryos for cryopreservation: features related to freezing tolerance
  • 3.. Conclusion
  • Chapter 5. Factors influencing cryopreservation of domestic animal embryos
  • 1.. Introduction
  • 2.. Factors affecting freezing tolerance
  • 3.. Cryobiological aspects
  • 4.. Possible methods to improve freezing resistance
  • 5.. Conclusion
  • Chapter 6. Timing of embryo development
  • 1.. Introduction
  • 2.. Timing of embryo development as an indicator of viability
  • 3.. Genetic regulation of embryo development timing
  • 4.. Timing of events during fertilization
  • 5.. Culture media effects on timing of embryo development
  • 6.. Timing of human embryo development
  • 7.. Summary and conclusions
  • Chapter 7. Biopsy and preimplantation diagnosis of human embryos
  • 1.. Introduction
  • 2.. Preimplantation genetic diagnosis (PGD)
  • 3.. Obtaining oocytes and embryos
  • 4.. Methods of oocyte and embryo biopsy
  • 5.. Methods of diagnosis
  • 6.. Use of PGD technology for evaluation of mammalian embryo quality
  • Chapter 8. Biopsying and genotyping cattle embryos
  • 1.. Introduction
  • 2.. Embryo biopsy methods
  • 3.. Pregnancy rates
  • 4.. DNA tests performed on the biopsy
  • 5.. Conclusion
  • Chapter 9. Multiphoton microscopy for imaging mammalian embryos
  • 1.. Introduction
  • 2.. Multiphoton microscopy basics
  • 3.. Embryo culture for imaging
  • 4.. Viability (laboratory species)
  • 5.. Imaging depth (domestic species)
  • 6.. Maximizing information from rare specimens (non-human primate)
  • 7.. Potential future directions
  • 8.. Conclusion
  • Chapter 10. Is the frequency of chromosome abnormalities influenced by in vitro techniques?
  • 1.. Introduction
  • 2.. Methodology
  • 3.. Chromosome abnormalities in embryos
  • 4.. Is the incidence of chromosomal abnormalities increased by in vitro culture?
  • 5.. Conclusion
  • Chapter 11. Differential staining of inner and outer cells to assess mammalian embryo quality
  • 1.. Introduction
  • 2.. Embryonic development
  • 3.. Differential staining of the blastocyst
  • 4.. Conclusion
  • Chapter 12. Apoptosis in mammalian embryos
  • 1.. Introduction
  • 2.. Definitions of cell death
  • 3.. Characteristic features of apoptosis in mammalian embryos: incidence and detection
  • 4.. Examples of application
  • 5.. Advantages and disadvantages of different techniques
  • 6.. Conclusions
  • Chapter 13. Electron microscopy of pre-hatching mammalian embryos: the ultrastructure of cattle, swine and horse embryos
  • 1.. Introduction
  • 2.. Basic considerations on the ultrastructure of pre-hatching mammalian embryos
  • 3.. Ultrastructure of the oocyte, zygote and pre-hatching embryo in cattle
  • 4.. Ultrastructure of the zygote and pre-hatching embryo in swine
  • 5.. Ultrastructure of the zygote and pre-hatching embryo in horse
  • Chapter 14. Differences in gene expression patterns related to origin of preimplantation embryos
  • 1.. Introduction
  • 2.. Methodological aspects of mRNA expression analysis
  • 3.. Gene expression patterns in bovine preimplantation embryos
  • 4.. Alterations in gene expression patterns of preimplantation rodent embryos
  • 5.. Gene expression patterns in human embryos
  • 6.. Summary and concluding remarks
  • Chapter 15. Protocols
  • Appendix I. The electron microscope
  • The transmission and scanning electron microscope
  • Microscopical resolution
  • Specimen preparation for transmission electron microscopy
  • Appendix II. Processing of eggs for transmission electron microscopy
  • Appendix III. Human preimplantation diagnosis pcr and RT-PCR
  • Appendix IV. Human preimplantation diagnosis fluorescence in situ-hybridization (FISH) on individual blastomeres
  • Appendix V. Fluorescence in situ-hybridization (FISH) on metaphases in bovine embryos
  • Appendix VI. Differential staining
  • Index
  • Colour plates
The Sumner Mayburg Library Fund, established with a bequest from her estate bookplate
The John Crerar Library bookplate

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