Animal transgenesis and cloning /
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| Author / Creator: | Houdebine, Louis-Marie. |
|---|---|
| Uniform title: | Transgenè€se animale et clonage. English |
| Imprint: | Chichester, UK ; Hoboken, NJ : John Wiley & Sons, c2003. |
| Description: | xiv, 220 p. : ill. ; 23 cm. |
| Language: | English |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/4887374 |
Table of Contents:
- Introduction
- Abbreviations and Acronyms
- 1. From the gene to the transgenic animal
- 1.1. Genome composition
- 1.2. Gene structure
- 1.3. The number of genes in genomes
- 1.4. The major techniques of genetic engineering
- 1.4.1. Gene cloning
- 1.4.2. DNA sequencing
- 1.4.3. In vitro gene amplification
- 1.4.4. Gene construction
- 1.4.5. Gene transfer into cells
- 1.5. The systematic description of genomes
- 1.6. Classical genetic selection
- 1.7. Experimental mutation in genomes
- 1.7.1. Chemical mutagenesis
- 1.7.2. Mutagenesis by integration of foreign DNA
- 1.7.3. Mutagenesis by transgenesis
- 2. Techniques for cloning and transgenesis
- 2.1. Cloning
- 2.1.1. The main steps of differentiation
- 2.1.2. Cloning by nuclear transfer
- 2.2. Gene therapy
- 2.2.1. The goals of gene therapy
- 2.2.2. The tools of gene therapy
- 2.2.3. The applications of gene therapy
- 2.3. Techniques of animal transgenesis
- 2.3.1. The aims and the concept of animal transgenesis
- 2.3.2. Gene transfer into gametes
- 2.3.3. Gene transfer into embryos
- 2.3.4. Gene transfer via cells
- 2.3.5. Vectors for gene addition
- 2.3.6. Vectors for gene replacement
- 2.3.7. Vectors for the rearrangement of targeted genes
- 2.3.8. Targeted integration of foreign genes
- 2.3.9. Non-classical vectors for the recombination of targeted genes
- 2.3.10. Vectors for gene trap
- 2.3.11. Vectors for the expression of transgenes
- 3. Applications of cloning and transgenesis
- 3.1. Applications of animal cloning
- 3.1.1. Basic research
- 3.1.2. Transgenesis
- 3.1.3. Animal reproduction
- 3.1.4. Human reproduction
- 3.1.5. Therapeutic cloning
- 3.1.6. Xenografting
- 3.2. Applications of animal transgenesis
- 3.2.1. Basic research
- 3.2.2. Study of human diseases
- 3.2.3. Pharmaceutical production
- 3.2.4. Xenografting
- 3.2.5. Breeding
- 4. Limits and risks of cloning, gene therapy and transgenesis
- 4.1. Limits and risks of cloning
- 4.1.1. Reproductive cloning in humans
- 4.1.2. Reproductive cloning in animals
- 4.1.3. Therapeutic cloning
- 4.2. Limits and risks of gene therapy
- 4.3. Limits and risks of transgenesis
- 4.3.1. Technical and theoretical limits
- 4.3.2. Biosafety problems in confined areas
- 4.3.3. The intentional dissemination of transgenic animals into the environment
- 4.3.4. The risks for human consumers
- 4.3.5. Transgenesis and animal welfare
- 4.3.6. Patenting of transgenic animals
- 4.3.7. Transgenesis in humans
- Conclusion and Perspectives
- References
- Index
