Lentivirus gene engineering protocols /
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| Imprint: | Totowa, N.J. : Humana Press, 2003. |
|---|---|
| Description: | xi, 314 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular biology ; v. 229 Methods in molecular biology (Clifton, N.J.) ; v. 229. |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/4921458 |
Table of Contents:
- Preface
- Contributors
- Part I.. Lentivirus Vectors
- 1. From Lentiviruses to Lentivirus Vectors
- 2. The Choice of a Suitable Lentivirus Vector: Transcriptional Targeting
- 3. Choice and Use of Appropriate Packaging Cell Types
- 4. Small- to Large-Scale Production of Lentivirus Vectors
- 5. Detection and Titration of Lentivirus Vector Preparations
- 6. Detection and Selection of Lentiviral Vector-Transduced Cells
- 7. Detection of Replication-Competent Lentiviral Particles
- Part II.. Transduction of Ex Vivo Cell Cultures Through Lentivirus Vectors
- 8. Lymphocytes
- 9. Monocyte/Macrophages and Dendritic Cells
- 10. Hematopoietic Stem and Progenitor Cells
- 11. Mesenchymal Stem Cells
- 12. Hippocampal Neurons
- 13. Dorsal Root Ganglia Sensory Neurons
- 14. Cardiomyocytes
- 15. Airway Epithelia
- 16. Corneal Cells
- 17. Retinal Tissue
- Part III.. Advantages and Uses of Non-HIV-1-Based Lentivirus Vectors
- 18. HIV-2 Vectors
- 19. SIV Vectors
- 20. FIV Vectors
- 21. Hybrid Lentivirus Vectors
- Part IV.. Direct In Vivo Transduction by Lentivirus Vector and Detection
- 22. Cells of Respiratory Epithelium
- 23. Cells of the Nervous System
- Index
