Molecular diagnosis of infectious diseases /
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| Edition: | 2nd ed. |
|---|---|
| Imprint: | Totowa, N.J. : Humana Press, c2004. |
| Description: | xv, 464 p. : ill. ; 24 cm. |
| Language: | English |
| Series: | Methods in molecular medicine ; 94 |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5056713 |
Table of Contents:
- Foreword
- Preface
- Contributors
- Part I.. Protein-Based Identification of Antigens
- 1. Proteomic Approaches to Antigen Discovery
- 2. Immunoproteomics
- 3. Immunoprecipitation and Blotting: The Visualization of Small Amounts of Antigens Using Antibodies and Lectins
- Part II.. DNA-Based Identification of Antigens
- 4. Representational Difference Analysis of cDNA
- 5. Microarray Data Analysis and Mining
- 6. Expression Cloning
- Part III.. Identification of Epitopes and Immunomodulatory Components
- 7. Determination of Epitopes by Mass Spectrometry
- 8. Identification of T Cell Epitopes Using ELISpot and Peptide Pool Arrays
- 9. Virus-like Particles: A Novel Tool for the Induction and Monitoring of Both T-Helper and Cytotoxic T-Lymphocyte Activity
- 10. Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components
- Part IV.. Expression of Recombinant Proteins
- 11. High-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well Format
- 12. Production of Antigens in Chlamydomonas reinhardtii: Green Microalgae as a Novel Source of Recombinant Proteins
- 13. Codon-Optimized Genes that Enable Increased Heterologous Expression in Mammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNA
- Part V.. Purification, Modification, and Renaturation of Recombinant Proteins
- 14. Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies)
- 15. Purification of Recombinant Proteins with High Isoelectric Points
- 16. Refolding of Inclusion Body Proteins
- 17. Small-Molecule-Protein Conjugation Procedures
- Part VI.. Characterization of Recombinant Proteins
- 18. Structural Characterization of Proteins and Peptides
- 19. Determination of Kinetic Data Using Surface Plasmon Resonance Biosensors
- 20. Affinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) Biosensor
- Part VII.. Evaluation of Recombinant Proteins in Immunological Test Systems
- 21. Solid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase Immunoassays
- 22. Design and Preparation of Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent Assays
- 23. Basic Problems of Serological Laboratory Diagnosis
- 24. Molecular Diagnostics Resources on the Internet
- Part VIII.. Recombinant Receptor Molecules
- 25. Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells
- Index
