Purifying proteins for proteomics : a laboratory manual /
Saved in:
| Imprint: | Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c2004. |
|---|---|
| Description: | xi, 801 p. : ill. (some col.) ; 29 cm. |
| Language: | English |
| Subject: | |
| Format: | Print Book |
| URL for this record: | http://pi.lib.uchicago.edu/1001/cat/bib/5082405 |
Table of Contents:
- Preface
- Purifying Proteins for Proteomics Companion Web Site
- Section I. Preparing Proteins for Proteomic Analysis
- 1. Role of Separation Science in Proteomics
- 2. Strategies for Purifying Proteins
- Section II. Chromatographic Methods for Preparing Proteins for Proteomics
- 3. Introduction to Chromatographic Methods for Protein and Peptide Purification
- 4. Multidimensional Chromatography of Intact Proteins
- 5. High-throughput Screening of Soluble Recombinant Proteins
- 6. Ion-exchange Chromatography
- 7. Size-exclusion Chromatography
- 8. Role of Reversed-phase High-performance Liquid Chromatography in Protein Isolation and Purification
- 9. Hydrophobic Interaction Chromatography
- 10. Affinity and Immunoaffinity Chromatography
- 11. Dye-Ligand Affinity Chromatography in Proteomic Research
- 12. Immobilized Metal-ion Affinity Chromatography
- 13. Protein Chromatography on Hydroxyapatite
- 14. Chromatofocusing
- Section III. Electrophoretic Methods for Preparing Proteins for Proteomics
- 15. Introduction to Strategies Based on Electrophoresis for Separating Proteins
- 16. Separation of Proteins Using Two-dimensional Gel Electrophoresis
- 17. Sample Preparation for High-resolution Two-dimensional Electrophoresis by Isoelectric Fractionation in an MCE
- 18. An Electrophoresis Method for Purifying Proteins: The Gradiflow BF400 Instrument
- 19. Protein Purification Using Free-flow Electrophoresis
- Section IV. Analysis of Purified Proteins prior to Structural Proteomics
- 20. Introduction to Methods for Characterizing Purified Proteins
- 21. Characterization of Proteins by Mass Spectrometry
- 22. Analytical Ultracentrifugation: Protein Size, Conformation, and Interactions
- 23. Measuring the Conformational Stability of a Protein
- 24. Combining Surface Plasmon Resonance with Mass Spectrometry: Identifying Binding Partners and Characterizing Interactions
- 25. Analysis of Carbohydrate from Glycoproteins
- Appendices
- 1.. An Overview of Protein Structure
- 2.. Measuring the Concentration of Proteins
- 3.. Concentrating Solutions of Protein
- 4.. Stabilization of Proteins for Storage
- 5.. One-dimensional Polyacrylamide Gel Electrophoresis
- 6.. Troubleshooting Guides
- HPLC
- 2D Gels
- 7.. Cautions
- Index
